换一批Oligonucleotide melting temperatures under PCR conditions: nearest-neighbor corrections for Mg(2+), deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas.
第一作者：
N,von Ahsen
第一单位：
Department of Clinical Chemistry, Georg-August University, Robert Koch Strasse 40, 37075 Goettingen, Germany. nahsen@gwdg.de
作者：
医学主题词
阳离子, 二价(Cations, Divalent);脱氧核糖核苷酸类(Deoxyribonucleotides);二甲亚砜(Dimethyl Sulfoxide);指示剂和试剂(Indicators and Reagents);镁(Magnesium);数学(Mathematics);寡核苷酸类(Oligonucleotides);聚合酶链反应(Polymerase Chain Reaction)
PMID
11673362
发布时间
2019-07-22
- 浏览130
Clinical chemistry
1956-61页
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