Metal-ion-dependent GFP emission in vivo by combining a circularly permutated green fluorescent protein with an engineered metal-ion-binding coiled-coil.
第一作者:
Toshihisa,Mizuno
第一单位:
Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan. toshitcm@nitech.ac.jp
作者:
主题词
氨基酸序列(Amino Acid Sequence);阳离子, 二价(Cations, Divalent);圆二色性(Circular Dichroism);绿色荧光蛋白质类(Green Fluorescent Proteins);金属, 重(Metals, Heavy);分子序列数据(Molecular Sequence Data);寡肽类(Oligopeptides);蛋白质工程(Protein Engineering);蛋白质折叠(Protein Folding);蛋白质结构, 二级(Protein Structure, Secondary);重组融合蛋白质类(Recombinant Fusion Proteins);光谱法, 荧光(Spectrometry, Fluorescence)
DOI
10.1021/ja0685102
PMID
17722917
发布时间
2007-09-12
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