换一批CTP: CMP phosphotransferase activity in L1210 cells.
摘要：
Substrate converting enzymes interfering with the measurement of ribonucleotide reductase were assessed in cell-free extracts prepared from L1210 cells. Data show the presence of a myokinase-type enzyme activity (CTP:CMP phosphotransferase) which catalyzes the reaction: 2CDP in equilibrium CMP + CTP. This enzyme is not removed by passage of cell extracts over ATP-agarose columns. Monitoring of nucleoside diphosphate substrate level is, therefore, mandatory for obtaining accurate measurements of CDP reductase activity in crude cell extracts.
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作者单位：
Department of Internal Medicine
[1]
University of South Florida College of Medicine
[2]
Tampa 33612.
[3]
期刊：
主题词：
动物(Animals);细胞提取物(Cell Extracts);色谱法, 琼脂糖(Chromatography, Agarose);色谱法, 高压液相(Chromatography, High Pressure Liquid);胞苷二磷酸(Cytidine Diphosphate);胞苷三磷酸(Cytidine Triphosphate);胞苷酸类(Cytosine Nucleotides);白血病L1210(Leukemia L1210);小鼠(Mice);磷酸转移酶类(Phosphotransferases)
PMID：
2840195
发布时间：
2007-11-14
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