Nature of the binding of activated cyclophosphamide to calf thymus DNA and pBR-322 plasmid DNA.
Differentially radiolabeled cyclophosphamide (CP) was metabolized with a reconstituted cytochrome P-450 system in the presence of calf thymus DNA or plasmid pBR-322 DNA. The isolated DNA was subjected to agarose gel electrophoresis and the associated radioactivity was determined. The majority (greater than 90%) of the initial DNA-bound radioactive metabolites dissociated from the DNA upon gel electrophoresis. The results are discussed in context to the binding of activated CP to DNA and the nature of the binding.更多