Energetics of the oxidase activity of NADPH-cytochrome P-450 reductase.
Oxidase activity of rat liver microsomes has been evaluated microcalorimetrically. delta H for the oxidation of reduced nicotine adenine dinucleotide phosphate was measured to be -39.33 (uncertainty 2.18) kcal/mol. The contribution of peroxide reduction and NADPH ring opening heats to this value have been considered. Calorimetric assay of the hydroxylase activity of the P-450-reductase-hydroxylase enzymatic complex has been demonstrated using the organocarbamate, Isolan (1-isopropyl-3-methyl-5-pyrazolyl dimethylcarbamate), as substrate.更多