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Effect of pH on fluorescence polarization of DMSO induced Friend leukemic cells.

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The effect of DMSO on Friend leukemic cell membranes was investigated in an inducible cell line (FLC) and in cell variant resistant to DMSO induction (RFLC). Fluorescence polarization (P) studies carried out at 37 degrees C with diphenyl hexatriene (DPH) have shown that P values change according to the pH, being maximum in alkaline buffer. These changes were altered when cells were grown in the presence of DMSO. Changes in P values according to the pH were abolished by glutaraldehyde and by sodium azide. Fluorescence polarization analysis of FLC cell line carried out at 4 degrees C have shown higher P values which also changed according to the pH. In DMSO induced FLC or in RFLC grown with or without DMSO, these changes were not seen. It is therefore concluded that P value changes related to pH reflect changes in the lipid-protein interactions of cell surface membrane. It is also suggested that these interactions are altered by DMSO.

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