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Plasma membranes were purified from tumor tissue of five malignant melanoma patients. The purified membranes were solubilized by sonication in KC1 in the presence of Triton X 100. The soluble membranes that were obtained by this procedure contained human melanoma tumor-specific antigens. The antigens were separated from the other soluble plasma membrane proteins by chromatography on affinity columns made from the patients' tumor-specific pre-absorbed IgG coupled to Sepharose 4B. Specific antigens which were eluted from these affinity columns with potassium thiocyanate were determined by crossover immunoelectrophoresis. The five tumors appear to have a similar complement of melanoma tumor-specific plasma membrane-associated antigens.