L-arginine binding to liver arginase requires proton transfer to gateway residue His141 and coordination of the guanidinium group to the dimanganese(II,II) center.
第一作者:
S V,Khangulov
第一单位:
Department of Chemistry, Henry H. Hoyt Laboratory, Princeton University, New Jersey 08544, USA.
作者:
主题词
动物(Animals);精氨酸酶(Arginase);精氨酸(Arginine);天冬酰胺(Asparagine);结合部位(Binding Sites);硼酸盐(Borates);瓜氨酸(Citrulline);电子自旋共振谱学(Electron Spin Resonance Spectroscopy);胍(Guanidine);组氨酸(Histidine);氢离子浓度(Hydrogen-Ion Concentration);异亮氨酸(Isoleucine);肝(Liver);赖氨酸(Lysine);锰(Manganese);诱变, 定点(Mutagenesis, Site-Directed);鸟氨酸(Ornithine);质子(Protons);大鼠(Rats);底物特异性(Substrate Specificity)
DOI
10.1021/bi972874c
PMID
9622506
发布时间
2016-10-19
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Biochemistry
8539-50页
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