2015年北京市房山区乙型Yamagata系流感病毒HA基因特性分析
Gene analysis of influenza B Yamagata lineage viruses in Fangshan district of Beijing in 2015
目的 了解北京市房山区2015年乙型Yamagata系流感病毒HA1基因变异情况.方法 选取2015年流感病原学监测中分离到的乙型Yamagata系毒株共13株,采用RT-PCR法扩增病毒HA基因片段后进行序列测定,与WHO推荐的疫苗株进行比对并构建进化树.采用MEGA6软件对测序结果进行分析.结果 2015年房山区乙型Yamagata系流感病毒HA1基因片段序列测定拼接后核苷酸长度为1059bp,编码氨基酸为353个,与2014-2015年的疫苗株B/Massachusetts/02/2012比较,13株毒株的氨基酸均在第123、131、165、180、187、196、211、217、244、313、327位点有变异;与2012-2013年的疫苗株B/Wisconsin/01/2010比较,13株毒株的氨基酸在第187、313、327均有变异.做进化树分析,房山区2015年分离到的乙型Yamagata系流感病毒与疫苗株B/Wisconsin/1/2010在同一个分支上,距离较近,与B/Massachusctts/2/2012不在一个分支上,距离较远.结论 2015年北京市房山区乙型Yamagata系流感病毒HA1基因在抗原决定簇区已发生变异,但疫苗株B/Wisconsin/01/2010有保护作用,应继续关注氨基酸的替换.
更多Objective To analyze the genetic characteristics of influenza B Yamagata lineage in Fangshan district of Beijing in 2015.Methods A total of 13 strains of influenza B Yamagata lineage viruses were randomly selected from the strains had isolated in 2015.HA1 gene of B influenza Yamagata lineage viruses was amplified by reverse transcription-poly-merase chain reaction (RT-PCR) and sequenced.The seauences were used for gene evolution analysis compared with vaccine strain sequence recommended by WTO with phylogenetic analysis in MEGA6 software.Homologous was calculated on-line in the BLAST website.Results The length of the nucleotide sequence of HA1 gene obtained by sequencing was 1059bp,encoding 353 amino acids.Compared with the vaccine strain B/Massachusetts / 02/2012 in 2014-2015,the amino acids sequences of the 13 strains were different at poisitions 123,131,165,180,187,196,2 1 1,217,244,313 and 327.Compared with the vaccine strain B/Wisconsin/01/2010 in 2012-2013,the amino acids sequences of the strains were different at the positions 187,313 and 327.Phylogenetic tree of HA1 gene sequences showed the influenza B Yamagata lineage vruses in Fangshan District of Beijing in 2015 were close to the vaccine strain B / Wisconsin/l/2010 and they are on the same branch.Conclusions HA1 gene of influenza B Yamagata virus in Fangshan District of Beijing has been mutated in the region of antigenic determinant in 2015,but the vaccine strain B/Wisconsin/01/2010 had a protective effect.We should continue to pay attention to amino acid replacements.
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