• 医学文献
  • 知识库
  • 评价分析
  • 中外期刊
  • 学位
  • 会议
  • 专利
  • 成果
  • 标准
  • 法规
  • 临床诊疗知识库
  • 中医药知识库
  • 机构
  • 作者
论文 期刊
高级检索

检索历史 清除

2006-2016年四川省急性弛缓性麻痹患者柯萨奇病毒A组4型基因特征分析

Genetic characteristics of coxsackievirus A4 from acute flaccid paralysis patients in Sichuan province from 2006 to 2016

摘要:

目的 了解2006-2016年四川省急性弛缓性麻痹(acute flaccid paralysis,AFP)患者柯萨奇病毒A组4型(coxsackie virus A4,CV A4)的基因特征.方法 收集2006-2016年四川省各县区级疾病预防控制中心采集的AFP患者粪便标本,共3 816份.用人横纹肌肉瘤细胞和转人脊灰病毒受体的小鼠肺细胞进行病毒分离,采用微量中和试验法确定病毒型别,应用RT-PCR对分离到的CV A4进行VP1区全序列测定,并进行序列比对和构建进化树.结果 3 816份粪便标本分离到病毒383株,分离率为10.0%.其中,获得最多的是CV A4,共17株,占所有分离株的4.4%.毒株来源患者的临床诊断分别为:肠道病毒感染8例,格林巴利综合征4例,心肌炎4例和脑炎1例.经测序鉴定15株CV A4为C基因型,2株为B基因型.17株CV A4之间核苷酸同源性为85.4%~98.3%,氨基酸同源性为94.7%~99.6%.结论 2006-2016年四川省AFP患者中分离到的CV A4以C基因型为主,同时也发现了罕见的B基因型.

更多
abstracts:

Objective To understand the genetic characteristics of Coxsackie virus A4 (CV A4) from acute flaccid paralysis (AFP) patients in Sichuan province from 2006 to 2016.Methods A total of 3 816 fecal specimens from AFP cases were collected by county and borough centers for disease control and prevention in Sichuan from 2006 to 2016.The virus strains were isolated by RD and L20B cells and were identified by micro-neutralization test.The complete VP1 genes were amplified by the reverse transcription-polymerase chain reaction (RT-PCR).The PCR products were sequenced directly and the sequences were aligned and the phylogenetic analysis was constructed.Results Three hundred and eighty-three strains were isolated from 3 816 fecal specimens and the isolation rate was 10%.The most common virus was CV A4 (17 isolates),accounting for 4.4% of all strains.8 strains were isolated from cases clinically diagnosed as enterovirus infection,4 strains were from cases with myositis,4 strains were from cases with peripheral neuritis and 1 strain was from cases with encephalitis.15 CV A4 strains were identified as genogroup C and 2 strains as genogroup B.The nucleotide and the amino acid identities among seventeen CVA4 strains were 85.4%-98.3% and 94.7%-99.6%,respectively.Conclusions The CV A4 strain with genogroup C was prevalent in Sichuan province during the period of 2006-2016,and CV A4 with genogroup B was the first-ever report in mainland China.

More
作者: 庞启迪 [1] 陈娜 [2] 马小珍 [2]
第一作者: 庞启迪
期刊: 《国际病毒学杂志》2018年25卷4期 251-254页 ISTIC
栏目名称: 论著
DOI: 10.3760/cma.j.issn.1673-4092.2018.04.011
发布时间: 2018-09-20
基金项目:
四川省卫生和计划生育委员会科研课题 The Science and Technology Project of SiChuan Health Planning Committee
  • 浏览:32
  • 下载:6

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!

扩展文献

特别提示:本网站仅提供医学学术资源服务,不销售任何药品和器械,有关药品和器械的销售信息,请查阅其他网站。

充值 订阅 收藏 移动端 使用
帮助