两种沙粒病毒核衣壳蛋白的交叉反应研究
Study on cross-reactivity between nucleocapsid proteins of two arenaviruses
目的 了解沙粒病毒的温州病毒(WENV)与淋巴细胞脉络丛脑膜炎病毒(LCMV)之间的抗原交叉反应情况.方法 分别利用原核细胞表达系统及昆虫细胞表达系统,对WENV、LCMV的核衣壳(nucleocapsid,N)蛋白进行表达、纯化;然后用纯化的原核细胞系统表达的蛋白免疫小鼠,制备抗血清.用纯化的昆虫细胞系统表达的蛋白作为检测抗原,与抗血清之间进行western-blot鉴定,分析WENV与LCMV N蛋白之间的交叉反应.结果 WENV与LCMV的N蛋白之间存在双向交叉反应.结论 LCMV与WENV的N蛋白之间存在抗原交叉反应,为进一步进行WENV的血清学研究提供重要的实验依据.
更多Objective To analyze the antigen cross-reactivity between wenzhou virus (WENV)and lymphocytic choriomeningitis virus (LCMV).Methods The nucleocapsid (N) proteins of WENV and LCMV were expressed in prokaryotic cell expression system and insect cell expression system and were purified.The purified N proteins derived from prokaryotic cell expression system were used to immunize the mice and antisern was prepared.N proteins derived from insect cell expression system was used as the antigen.Western-blot was used to test the cross-reactivity between N proteins of WENV and LCMV.Results The antigen cross-reactivity was detected between N proteins of WENV and LCMV.Conclusions The cross-reactivity existed between N proteins of WENV and LCMV.This finding provides an experimental basis for further serological study of WENV.
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