连续量化外周血淋巴细胞染色体的制备方法
Continuous quantitative preparation methods of chromosome of peripheral blood lymphocytes
目的 快速制备外周血淋巴细胞染色体,提高分析效率,为染色体制备的规范化标准化提供理论依据.方法 连续量化法在传统制备方法基础上去掉三次室温放置固定时间,滴片后随之烤片,提高秋水仙素终浓度(0.008 μg/mL)等方面进行了优化,同时与传统制备法进行对比.结果 278例染色体标本:传统组400~600条分裂相占63%,连续量化收获组占65%(P=0.065);传统组染色体分散良好率65%,连续量化收获组良好率66%(P=0.088);两组比较无显著差异,表明两种方法无差别.结论 连续量化外周血淋巴细胞染色体的制备方法重复性和一致性好,缩短了染色体制备过程,提高了染色体分析的效率.
更多Objective In order to provide theoretical basis for the standardization of chromosome preparation, we improve the efficiency of nuclear analysis by rapidly preparing the peripheral blood lymphocyte.Methods continuous quantitative method is optimized in the fixed time, baking and colchicine concentration, removing three room placed times fixed time based on the traditional preparation methods, Baking pieces immediately after the drop and improving the colchicine concentration to 0.008 μg/mL, compared with the traditional preparation method.Results Results of 278 chromosome specimens: 63% of the traditional group of 400~600, and 65% of continuous quantification group (P=0.065);The dispersion of chromosomes in the traditional group was 65%, and the good rate of the continuous quantification group was 66% (P=0.088).There was no significant difference between the two groups, indicating that there was no difference between two methods.Conclusion continuous quantitative of peripheral blood lymphocyte chromosome preparation method had good repeatability and consistency, shorten the chromosome preparation process and improved the efficiency of the chromosome analysis.
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