您的账号已在其他设备登录,您当前账号已强迫下线,
如非您本人操作,建议您在会员中心进行密码修改

确定

影响新生儿氨基酸代谢的多因素分析

Multivariate analyses of factors that affect neonatal screening amino acids

摘要:

目的:探讨新生儿体内氨基酸变化特点,为临床精准判读提供理论依据。方法:在2018年1月至12月四川地区采用串联质谱技术进行遗传代谢病(IMD)筛查的32 855例新生儿中,纳入经初筛、复查确定为未患IMD的32 843例新生儿。根据胎龄(早产儿1 363例,足月儿31 468例,过期产儿12例)、采血时间(日龄3~7 d采血30 958例、8~28 d采血1 637例、>28 d采血248例)、出生季节(第1季度出生7 737例、第2季度出生11 428例、第3季度出生5 482例、第4季度出生8 196例)进行分组,比较各组新生儿体内氨基酸的水平差异,分析多种影响因素与代谢指标的相关性。结果:1.新生儿体内11种氨基酸[丙氨酸(ALA)、精氨酸(ARG)、瓜氨酸(CIT)、甘氨酸(GLY)、亮氨酸+异亮氨酸+羟脯氨酸(LEU+ILE+PRO-OH)、蛋氨酸(MET)、鸟氨酸(ORN)、苯丙氨酸(PHE)、脯氨酸(PRO)、酪氨酸(TYR)、缬氨酸(VAL)]均呈非正态分布。2.按胎龄分组,除PHE( H=0.61, P>0.05)、TYR( H=2.02, P>0.05)差异无统计学意义外,ALA( H=187.11, P<0.05)、ARG( H=23.60, P<0.05)、CIT( H=22.90, P<0.05)、GLY( H=85.18, P<0.05)、LEU( H=56.42, P<0.05)、MET( H=18.74, P<0.05)、ORN( H=129.27, P<0.05)、PRO( H=344.40, P<0.05)、VAL( H=272.92, P<0.05)差异均有统计学意义。3.按采血时间分组,ALA( H=65.19, P<0.05)、ARG( H=404.48, P<0.05)、CIT( H=502.13, P<0.05)、GLY( H=1 719.44, P<0.05)、LEU( H=396.41, P<0.05)、MET( H=199.39, P<0.05)、ORN( H=31.26, P<0.05)、PHE( H=325.49, P<0.05)、PRO( H=70.09, P<0.05)、TYR( H=159.29, P<0.05)、VAL( H=102.52, P<0.05)差异均有统计学意义。4.按出生季节分组,ALA( H=401.37, P<0.05)、ARG( H=3 229.03, P<0.05)、CIT( H=65.45, P<0.05)、GLY( H=597.82, P<0.05)、LEU( H=1 120.42, P<0.05)、MET( H=10 515.18, P<0.05)、ORN( H=1 275.23, P<0.05)、PHE( H=2 260.17, P<0.05)、PRO( H=319.57, P<0.05)、TYR( H=884.37, P<0.05)、VAL( H=1 824.49, P<0.05)差异均有统计学意义。 结论:新生儿体内氨基酸根据不同胎龄、出生季节和采血时间,呈不同的代谢差异,采用串联质谱技术检测时应根据不同条件选择适宜的判读标准。

更多
abstracts:

Objective:To explore the change characteristics of amino acid levels in neonates, so as to provide theoretical basis for accurate clinical interpretation.Methods:By preliminary screening and diagnosis from 32 855 newborns, 32 843 samples were collected using tandem mass spectrometry to inherited metabolic disease (IMD) scree-ning in Sichuan Province from January to December 2018.Afterwards, according to gestational age (1 363 premature infants, 31 468 full-term infants and 12 overdue infants), blood collection time (3-7 days old, 3 095 cases, 8-28 days old, 1 637 cases, and more than 28 days old, 248 cases) and season (7 737 cases in the first quarter, 11 428 cases in the second quarter, 5 482 cases in the third quarter, and 8 196 cases in the fourth quarter), neonates were divided into different study groups.The difference of amino acid level in each group was compared, and the correlation between various influencing factors and metabolic index was analyzed.Results:(1) The distribution of 11 amino acids [alanine(ALA), arginine(ARG), citrulline(CIT), glycine(GLY), leucine+ isoleucine+ hydroxyproline (LEU+ ILE+ PRO-OH), methionine(MET), ornithine(ORN), phenylalanine(PHE), proline(PRO), tyrosine(TYR), and valine(VAL)] in neonates showed non-normally distribution.(2)The distribution of 11 amino acids in different gestational age were tested by nonparametric test, except for PHE( H=0.61, P>0.05)and TYR( H=2.02, P>0.05), and other indicators were significantly different [ALA( H=187.11, P<0.05), ARG( H=23.60, P<0.05), CIT( H=22.90, P<0.05), GLY( H=85.18, P<0.05), LEU( H=56.42, P<0.05), MET( H=18.74, P<0.05), ORN( H=129.27, P<0.05), PRO( H=344.40, P<0.05), and VAL( H=272.92, P<0.05)]. (3) The distribution of 11 amino acids in different blood collection time were significantly different [ALA( H=65.19, P<0.05), ARG( H=404.48, P<0.05), CIT( H=502.13, P<0.05), GLY( H=1 719.44, P<0.05), LEU( H=396.41, P<0.05), MET( H=199.39, P<0.05), ORN( H=31.26, P<0.05), PHE( H=325.49, P<0.05), PRO( H=70.09, P<0.05), TYR( H=159.29, P<0.05), and VAL( H=102.52, P<0.05)]. (4) The distribution of 11 amino acids in different birth seasons were significantly different [ALA( H=401.37, P<0.05), ARG( H=3 229.03, P<0.05), CIT( H=65.45, P<0.05), GLY( H=597.82, P<0.05), LEU( H=1 120.42, P<0.05), MET( H=10 515.18, P<0.05), ORN( H=1 275.23, P<0.05), PHE( H=2 260.17, P<0.05), PRO( H=319.57, P<0.05), TYR( H=884.37, P<0.05), and VAL( H=1 824.49, P<0.05)]. Conclusion:According to different gestational age, season and blood collection time, the metabolism of amino acids in neonates was different.When using tandem mass spectrometry for detection, appropriate interpretation criteria should be selected based on different conditions.

More
作者: 周婧瑶 [1] 张钰 [1] 胡琦 [1] 陈雪莲 [1] 杨丽涓 [1] 张亚果 [1] 苏星月 [1] 杨云霞 [1] 欧明才 [1]
期刊: 《中华实用儿科临床杂志》2020年35卷23期 1773-1776页 ISTICPKUCA
栏目名称: 新生儿疾病
DOI: 10.3760/cma.j.cn101070-20200109-00042
发布时间: 2021-01-11
  • 浏览:25
  • 下载:15

加载中!

相似文献

  • 中文期刊
  • 外文期刊
  • 学位论文
  • 会议论文

加载中!

加载中!

加载中!

加载中!