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目的 检测复发性自然流产(RSA)患者外周血自然杀伤(NK)细胞的免疫表型。方法 应用流式细胞术检测27例RSA妊娠患者、41例RSA非妊娠患者、32例正常妊娠者和25例正常非妊娠者外周血CD56dimCD 16+、CD56brightCD16+/-、CD69+、HLA-DR+ NK细胞表达并进行比较。结果RSA妊娠组、RSA非妊娠组、正常妊娠组和正常非妊娠组外周血CD56dimCD16+ NK细胞占总NK细胞的比例分别为(88.69.±5.86)％、(79.25±9.31)％、(79.24±10.09)％、(75.49±11.96)％;CD56brightCD16+/- NK细胞的比例分别为(8.18±5.54)％、( 12.20±6.49)％、( 13.13±8.65)％、( 11.53±6.23)％；CD69+ NK细胞的比例分别为(3.42±2.13)％、(2.36±1.72)％、(2.68±1.81)％、(2.08±1.73)％；HLA-DR+ NK细胞的比例分别为(15.26±9.32)％、(9.96±7.18)％、( 10.57±8.05)％、(9.64±6.12)％。与RSA非妊娠组、正常妊娠组及正常非妊娠组比较，RSA妊娠组CD56dimCD16+、CD69+、HLA-DR+ NK细胞显著升高（均P＜0.05）。与RSA非妊娠组及正常妊娠组比较，RSA妊娠组CD56brightCD16+/- NK细胞明显降低（均P＜0.05）。结论 RSA妊娠患者外周血中NK细胞CD56dimCD16+、CD69+和HLA-DR+表达增高，而CD56brightCD16+/-表达下降，可能在RSA的发生发展中起着一定的作用。

Objective To determine the immunophenotypes of peripheral nature killer (NK) cells in patients with recurrent spontaneous abortion (RSA). Methods Flow cytometry was used to determine the expressions of CD56dimCD16+, CD56brightD16+/-, CD69+ and HLA-DR+ NK cells in peripheral blood samples from 27 pregnant and 41 non-pregnant RSA patients, 32 normal pregnant and 25 non-pregnant women, and the expressions were compared among 4 groups. Results The percentage of total peripheral NK cells from pregnant and non-pregnant RSA women, normal pregnant and non-pregnant women was (88.69± 5.86)％, (79.25±9.31 )％, (79.24±10.09)％ and (75.49±11.96)％, respectively, for CD56dimCD16+ NK cells; (8.18 ± 5.54)％ , (12.20 ± 6.49)％ , (13.13 ± 8.65)％ and (11.53 ± 6.23)％ , respectively, for CD56brightCD16+/- NK cells; (3.42±2.13)％ , (2.36± 1.72)％, (2.68± 1.81)％ and (2.08± 1.73)％ ,respectively, for CD69+ NK cells; (15.26±9.32)％, (9.96±7.18)％, (10.57±8.05)％ and (9.64±6.12)％,respectively, for HLA-DR+ NK cells. Compared with non-pregnant RSA women, normal pregnant or non-pregnant women, pregnant RSA women showed significantly higher percentage of CD56dimCD16% CD69+ and HLA-DR+ NK cells (all P＜0.05). Compared with non-pregnant RSA and normal pregnant women, pregnant RSA women showed significantly lower percentage of CD56brightCD16+/- NK cells (both P＜0.05).Conclusions The increased expression of CD56dimCD16+, CD69+ and HLA-DR+ NK cells and decreased expression of CD56brightCD16+/- NK cells in peripheral blood of the RSA pregnant patients may play a role in the development and progression of RSA.