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目的 观察人参皂苷Rg3对逆转人肝癌细胞BEL-7402耐药作用.方法 MTT法测定人参皂苷Rg3与长春新碱(VCR)联合对肝癌细胞BEL-7402生长抑制率,反转录多聚酶链反应(RT-PCR)检测人参皂苷Rg3作用于肝癌细胞MDRImRNA的表达量.结果 不同浓度的人参皂苷Rg3( 10、20、30、40、60 mg/L)与长春新碱(0.02 mg/L)联合作用后,其对细胞增殖抑制率较单独用药时作用增加(P＜0.05),相互作用指数CDI＜1.人参皂苷Rg3 40.0μg/mL作用于肝癌细胞BEL-7402不同时间(24、48、72h)后MDR1mRNA的表达量明显下降[(79.21±2.23)％,(62.58±2.46)％,(45.46±1.13)％],与对照组差异有统计学意义(P＜0.05).结论 人参皂苷Rg3与长春新碱联合应用具有协同抑制作用,能逆转BEL7402对VCR的耐药.人参皂苷Rg3能抑制BEL-7402细胞MDR1 mRNA的表达.

Objective To determine the effect of ginsenoside Rg3 on reversal of multidrug resistance of hepatocellular carcinoma (HCC) cells bel-7402.Methods MTT method was employed to determine the inhibitory rate of ginsenoside Rg3 in combination with vincristine(VCR) on human HCC cells bel-7402.The cffcct of ginscnoside Rg3 on MDR1 mRNA cxpression in HCC cells was detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results The ginsenoside Rg3 of various concentrations ( 10,20,30,40,60 mg/L) combined with VCR(0.02 mg/L) produced longer inhibitory action than either drug alone (all P＜0.05,CDI＜1).Ginsenoside Rg3 (40.0 mg/L) led to marked reduction in MDR1 mRNA expression[ (79.21±2.23)％,(62.58±2.46％,(45.46±1.13％) ] in HCC cells bel-7402 at various time points (24 h,48 h and 72 h) as compared with control group (all P＜0.05).Conclusion Charactcrizcd by inhibiting MDR1 mRNA expression in bel-7402 cells,ginsenoside Rg3 combined with VCR has co-inhibition on reversal of HCC cells bel-7402 resistance to VCR.