摘要Objective: To study the antimicrobial activity and phytochemical characterization of essential oil isolated from the rhizome of Curcuma xanthorrhiza against pathogenic bacteria and fungi.Methods:Fresh rhizomes of Curcuma xanthorrhiza were subjected to hydro distillation process to obtain essential oil and characterized by Gas Chromatography- Mass Spectroscopy (GC-MS). The essential oil was evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and six fungi by the disc diffusion method. Results: GC – MS analysis of the essential oil extracted from the rhizome of Curcuma xanthorrhiza contained the derivatives of xanthorihizol, camphene and curcumene, monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene, hydrocarbons and other minor compounds. The antimicrobial activity of the oil showed significant inhibitory activity against the human pathogenic bacteria, no activity was observed against the fungi Aspergillus niger and Fusarium oxysporum. Conclusions: The findings of the present study indicate that the rhizome extract of Curcuma xanthorrhiza possess secondary metabolites and potential to develop antimicrobial drugs.