紫杉醇对子宫癌患者血清及组织相关因子的影响
Influence of paclitaxel on the correlation factors in serum and tissue of patients with metrocarcinoma
目的:探讨紫杉醇对子宫癌患者血清及组织缺氧诱导因子-1α( HIF-1α)、血管内皮生长因子( VEGF)等相关因子的影响。方法回顾性分析48例子宫癌患者的临床资料,采用紫杉醇联合化疗方案对其进行治疗,在治疗前后,采用elisa法检测患者血清中HIF-1α和VEGF的含量,采用免疫组织化学法对患者的宫颈组织进行染色,观察HIF-1α和VEGF的表达情况,western bloting法检测宫颈组织中HIF-1α和VEGF表达水平的变化,并比较发生淋巴转移和未发生淋巴转移患者的HIF-1α和VEGF表达的改变。结果未发生淋巴转移子宫癌患者血清中HIF-1α和VEGF的含量分别为(16.71±34.75)ng/L、(255.77±87.99)ng/L,治疗后分别为(98.33±32.17)ng/L、(153.67±65.45)ng/L,治疗前后差异均有统计学意义(t=9.416、5.001, P=0.003、0.007);发生淋巴转移的子宫癌患者血清中HIF-1α和VEGF的含量升高,与未发生转移患者比较差异均有统计学意义( t=3.055、16.196,P=0.038、0.000);治疗后HIF-1α和VEGF含量下降;宫颈癌患者在接受治疗后,宫颈组织HIF-1α和VEGF的表达水平均明显下降,与治疗前比较差异有统计学意义( t=5.899, P=0.004;t=9.649,P=0.001);发生淋巴转移的患者在接受治疗后,HIF-1α的表达水平下降,与治疗前比较差异有统计学意义(t=5.233,P=0.004),但VEGF的表达未见明显变化。结论紫杉醇抗子宫癌的作用机制可能与抑制HIF-1α与VEGF蛋白的表达有关,进而抑制肿瘤组织血管生成和癌细胞转移。
更多Objective To explore the level of hypoxia-inducible factor-1α( HIF-1α) and vascular endotheli-al growth factor( VEGF) in serum and tissue of patients with metrocarcinoma.Methods 48 patients diagnosed with cervical cancer were analyzed retrospectively.Patients were treated with paclitaxel combination chemotherapy,HIF-1αand VEGF levels in serum of patients were detected by ELISA method before and after the treatment.HIF-1αand VEGF levels in cervical tissue of patients were observed by immunehistochemical staining methods.Western blot method was used to detect the change of HIF-1αand VEGF expression levels in cervical tissues of patients.And the levels of HIF-1αand VEGF in patients with lymphatic metastasis and lymph node metastases were compared. Results Lymph node metastasis did not occur in patients with uterine cancer serum HIF-1αand VEGF were (16.71 ±34.75)ng/L,(255.77 ±87.99)ng/L,respectively,and those after treatment were (98.33 ±32.17)ng/L, (153.67 ±65.45)ng/L,the differences were statistically significant(t=9.416,5.001,P=0.003,0.007) before and after treatment;The serum of patients with lymph node metastasis of uterine cancer in HIF-1αand VEGF increased, and cervical cancer patients after treatment,cervical tissue HIF-1αand VEGF;The metastasis were statistically signif-icant differences occurred(t=3.055,16.196,P=0.038,0.000);HIF-1αand VEGF decreased after treatment,the expression levels were significantly decreased compared with before treatment ( t=5.899,P=0.004;t=9.649,P=0.001);patients with lymph node metastasis after treatment,the expression levels of HIF-1αdecreased compared with before treatmen ( t =5.233, P =0.004), but there was no significant changes in the expression of VEGF. Conclusion The mechanism of taxol anti-uterine cancer may be related with inhibition the expression of HIF-1αand VEGF protein,and thereby inhibiting tumor angiogenesis and cancer metastasis.
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