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肝细胞癌组织中膜联蛋白A10表达及与基质金属蛋白酶-9、血管内皮生长因子相关性研究

The expression of annexin A10 in hepatocellular carcinoma and its correlation with matrix metalloproteinase 9 and vascular endothelial growth factor

摘要:

目的 检测膜联蛋白A10(ANXA10)在人肝细胞癌(HCC)组织中的表达水平,分析其与基质金属蛋白酶-9(MMP-9)和血管内皮生长因子(VEGF)的相关性,为临床诊断、评估HCC提供依据.方法 选择HCC患者88例,均进行手术治疗,HCC癌组织Ⅰ期11例、Ⅱ期25例,Ⅲ期31例、Ⅳ期21例,应用免疫组织化学法检测患者HCC癌组织、癌旁肝组织和正常肝组织中ANXA10、MMP-9、VEGF的表达,分析ANXA10与MMP-9、VEGF之间相关性.结果 HCC癌组织ANXA10吸光度值为(0.074±0.012),低于癌旁肝组织的(0.091±0.013)和正常肝组织的(0.131 ±0.025),且癌旁肝组织ANXA10表达低于正常肝组织,差异均有统计学意义(t=8.96、9.44、8.71,均P<0.05);HCC癌组织MMP-9和VEGF吸光度值分别为(0.147 ±0.017)和(0.127 ±0.028),高于癌旁肝组织的(0.096 ±0.012)和(0.091 ±0.015)、正常肝组织的(0.075±0.014)、(0.077 ±0.019),且癌旁肝组织MMP-9和VEGF表达高于正常肝组织,差异均有统计学意义(t=8.05、9.30、8.11,8.28、9.51、8.02,均P<0.05);Ⅲ期+Ⅳ期HCC癌组织ANXA10吸光度值为(0.056 ±0.010),低于Ⅰ期+Ⅱ期的(0.082 ±0.016),差异有统计学意义(t=8.90,P<0.05),Ⅲ期+Ⅳ期HCC癌组织MMP-9和VEGF吸光度值为(0.157±0.022)和(0.169±0.033),高于Ⅰ期+Ⅱ期的(0.114±0.015)和(0.091±0.021),差异均有统计学意义(t=9.13、9.72,均P<0.05).ANXA10与MMP-9和VEGF具有显著相关性(r=0.324,0.295,均P<0.05).结论 ANXA10在肝细胞癌组织中呈现低表达,并随着分期的增加表达下降,与MMP-9和VEGF呈现负相关,ANXA10表达缺失或失活可能是恶变的人肝细胞癌的重要特征之一.

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abstracts:

Objective To explore the expression of annexin A10 (ANXA10) in human hepatocellular carcinoma (HCC),and analyze its correlation with matrix metalloproteinases-9 (MMP-9) and vascular endothelial growth factor (VEGF),thus to provide the basis for clinical diagnosis and assessment of HCC.Methods 88 HCC patients were selected,and they were all performed surgical treatment,HCC stage Ⅰ in 11 cases,stage Ⅱ 25 cases,stage Ⅲ 31 cases,stage ⅣV 21 cases.The expressions of ANXA10,MMP-9,VEGF of HCC cancer tissues,adjacent liver tissues and normal liver tissues were tested by immunohistochemical method.The correlation of ANXA10 with VEGF and MMP-9 was analyzed.Results The absorbance value of ANXA10 expression in the HCC cancer tissue was (0.074 ± 0.012),which was lower than that in the adjacent liver tissues [(0.091 ± 0.013)] and normal liver tissues[(0.131 ±0.025)],and ANXA10 expression of the adjacent liver tissue was lower than that of the normal liver tissues,the differences were statistically significant (t =8.96,9.44,8.71,all P < 0.05).The absorbance values of MMP-9 and VEGF expression in the HCC cancer tissue were (0.147 ± 0.017) and (0.127 ± 0.028),respectively,which were higher than those in the adjacent liver tissues [(0.096 ± 0.012),(0.091 ± 0.015)] and normal liver tissues [(0.075 ± 0.014),(0.077 ± 0.019)].The absorbance values of MMP-9 and VEGF of the adjacent liver tissues were higher than those of the normal liver tissues,the differences were statistically significant (t =8.05,9.30,8.11;8.28,9.51,8.02,all P < 0.05).The absorbance value of ANXA 10 expression in the HCC stage Ⅲ + Ⅳ cancer tissue was (0.056 ± 0.010),which was lower than that in the stage Ⅰ + Ⅱ cancer tissue [(0.082 ±0.016)],the difference was statistically significant (t =8.90,P < 0.05).The absorbance values of MMP-9 and VEGF expression in the stage Ⅲ + Ⅳ cancer tissue were (0.157 ± 0.022) and (0.169 ± 0.033),respectively,which were higher than those in the stage Ⅰ + Ⅱ cancer tissue [(0.114 ±0.015),(0.091 ±0.021)],the differences were statistically significant (t =9.13,9.72,all P < 0.05).ANXA10 was correlated with MMP-9 and VEGF (r =0.324,0.295,all P < 0.05).Conclusion ANXA10 presents lower expression in HCC cell,and the expression decreased with the increase of staging.It is negatively related with the MMP-9 and VEGF.ANXA10 expression missing or inactivation of malignant change may be one of the most important features in HCC.

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