支气管哮喘IgE水平与β2-肾上腺素能受体基因多态性关系的研究
Study on relationship between serum total IgE and β《,2》-adrenoreceptor polymorphism in asthmatic patients
目的 探讨贵州汉族支气管哮喘人群血清总IgE水平与β2-肾上腺素能受体(β2-AR)16、27位点基因多态性的相关性.方法 44例支气管哮喘患者采用ELISA方法检测血清总IgE水平、等位基因特异性聚合酶链反应(AS-PCR)方法进行β2>-AR基因多态性分析.结果 β2-AR16位点的基因型Arg/Arg和Gly/Gly分布频率在IgE水平正常哮喘组、IgE水平升高哮喘组分别为17.4%、23.8%和43.5%、52.4%,呈递增趋势;Arg/Gly分布频率在两组中分别为39.1%、23.8%,呈递减趋势,但统计学分析差异无统计学意义;在27位点中,血清IgE水平升高的哮喘患者中,Gin/Gin纯合子所占比例明显增高,达到76.2%;血清IgE水平不升高的哮喘患者中,Gln/Glu杂合子所占比例明显增高,达到73.9%;2组比较差异有统计学意义(P<0.01).结论 血清总IgE水平与β2-AR27位点基因多态性相联,有助于贵州汉族支气管哮喘人群发病机制的进一步研究.
更多Objective To study the association between serum total IgE level and β2-adrenoreceptor polymorphism (β2-AR) in asthmatic patients. Methods ELISA was used to determine serum total IgE and AS-PCR was used to determine β2-adrenoreceptor polymor- phism in 44 asthmatic patients. Results In β2-AR 16 locus genotype, the distribution frequencies of Arg/Arg, Gly/Gly showed increasing tendency, whereas Arg/Gly showed decreasing tendency, in normal serum total IgE group and increased serum total IgE group. But there was no significant difference between this two groups. In β2- AR 27 locus genotype, the distribution frequency of Gln/Gln genotype accounts for 76.2% and Gln/Glu genotype for 19.0% in increased serum total IgE group, while Gln/Gln genotype accounts for 9.0% and Gln/Glu geno- type for 73.9% in normal serum total IgE group. There was significant difference between two groups ( P<0.01 ). Conclusions Serum total IgE was correlated with β2-AR 27 locus genetic polymorphism, which may contribute to understand the mechanism of asthma in the peo- ple of the Han nationality in GuiZhou.
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