2型糖尿病合并大血管病变患者外周血单核细胞中核因子-κB的活性变化及相关性研究
Studies of Nuclear Factor-κB in peripheral mononuclear cell of type 2 diabetes patients with macrovascular complications
目的 通过比较2型糖尿病(T2DM)合并大血管病变患者和无大血管病变病人的外周血单核细胞中核因子κB(NF-κB)的表达水平,探讨糖尿病病人外周血单核细胞中NF-κB的水平与糖尿痛大血管病变发生发展的关系.结果 分别采用免疫组织化学染色法和夹心酶联免疫吸附法测定50例2型糖尿病合并大血管病变患者(G3组),41例2型糖尿病无大血管病变患者(G2组)及30例对照组(G1组)的外周血单核细胞NF-κB活性和血浆可溶性细胞间黏附分子-1(sICAM-1)水平,并分析NF-κB活性与sICAM-1浓度相关性.结果 G3组外周血单核细胞中NF-κB活性和血浆sICAM-1水平显著高于G2组、G1组(P<0.01),G2组NF-κB活性和sICAM-1水平显著高于G1组(P<0.01),G3、G2、G1组外周血单核细胞中NF-κB活性与血浆sICAM-1水平呈正相关(r=0.376,0.283,0.302,P<0.05).结论 糖尿病大血管病变患者外周血单核细胞中核因子NF-κB表达的增加,可能通过上调sICAM-1表达参与了糖尿病周围大血管病变的发生发展过程.
更多Objective To study the relationship between NF kappa-B (NF-κB) and the development of diabetic macroangiopathy in patients with type 2 diabetes, the expression level of Nuclear Faetor-κB in peripheral mononuclear cell of type 2 diabetes patients with and without macroangiopathy were compared, Methods Enzyme-linked immunosorbent assay was used to measure soluble intercellular adhesion molecule-1 (slCAM-1) in plasma and immunohistochemical method was used to measure the activity of NF-κB in peripheral blood mononu-clear cell in 50 type 2 diabetes patients with macroangiopathy(G3) , 41 type 2 diabetes patients without maeroangiopathy(G2) and 30 nor-real control subjects(G1). Linear correlation model was used to analyze the correlation between the level of sICAM-1 and NF-κB. Results The expression level of Nuclear Factor-κB in peripheral mononuclear cell of G3 was significantly higher than that in G2 and G1(P <0.01). The concentration of plasma sICAM-1 in G3 was significantly higher than that in G2 and G1. The expression level of Nuclear Factor-κB in peripheral mononuclear cell of G2 was significantly higher than that in GI (P < 0.01). The concentration of plasma sICAM-1 in G2 was significantly higher than that in G1. The levels of plasma sICAM-1 in three groups were positive correlated with activity of NF-κB in peripher-al blood mononuclear cells(r = 0.376, 0.283, 0.302, P < 0.05). Conclusion Increased level of NF kappa-B in peripheral blood mono-nuclear might be risk factor for peripheral macroangiopathy in patients with diabetes. It may induce macroangiopathy via up-regulating sI- CAM-1 expression.
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