肺结核患者细胞免疫功能与CD4+T淋巴细胞凋亡相关性研究
The detection of relationship between cellular immune function and CD4 + T lymphocytes apoptosis in patients with pulmonary tuberculosis
目的 检测肺结核患者外周血CD4+T淋巴细胞凋亡及免疫功能状态,探讨其与结核病发病的临床意义.方法 分离结核病患者和正常人外周血单个核细胞,标记后用流式细胞仪测定CD4+T淋巴细胞凋亡率;用链霉亲和素碱磷酶法(SAP法)检测其外周血CD3+T、CD4+T、CD8+T淋巴细胞及CD4/CD8比值;采用酵母菌花环法检测其红细胞受体花环率及红细胞免疫复合物花环率.结果 结核病患者外周血CD4+T淋巴细胞凋亡率(15.882 ±4.65)%、CD8+T淋巴细胞比例(27.69±0.74)%、红细胞免疫复合物阳性率(19.40±0.58)%显著高于对照组(P<0.01),CD3+T淋巴细胞比例(46.48±1.34)%、CD4+T淋巴细胞比例(28.12±0.69)%,CD4/CD8比值(1.0223±0.09362)和红细胞C3b受体阳性率(17.73±0.63)%明显降低(P<0.01);结核病患者CD4+T淋巴细胞凋亡率与CD4+T淋巴细胞比例之间以及T淋巴细胞亚群与红细胞免疫功能之间存在一定的相关性.结论 结核病患者CD4+T淋巴细胞凋亡率显著增加致CD4+T淋巴细胞数减少,T淋巴细胞免疫功能与红细胞免疫功能存在正相关,且均有所下降,可能与肺结核的免疫发病有关.
更多Objective To study the apoptosis of CD4 + T lymphocytes and the detection of immune function in patients with pulmonary tuberculosis and explore the clinical significance.Methods The mononuclear cells were separated from the blood of the tuberculosis patients or the healthy.The flow cytometry was used to measure the percentage of apoptotic CD4 + T lymphocytes,and the standard of T-lymphocyte subsets were detected by using SAP technology.The red cell immune function were determined by using yeast wreath way.Results The apoptosis rate of CD4 + T lymphocytes and CD8 + T lymphocyte was ( 15.882 ± 4.65 ) %,and (27.69 ± 0.74) %.The Immune complex positive rate ( 19.40 ± 0.58) % in patients with tuberculosis was significantly higher than those in controls ( P < 0.01 ).C3b receptor positive rate in red blood cells was ( 17.73 ± 0.63 ) %,( 46.48 ± 1.34 ) % in CD3 + T lymphocyte,( 28.12 ±0.69 ) % in CD4 + T lymphocyte,and the ratio of CD4/CD8 ( 1.0223 ± 0.09362) in the patients with tuberculosis was lower than the control group( P < 0.01 ).There were certain relationships between the apoptosis rate of CD4 + T lymphocytes and the percentages of CD4 + T lymphocyte,the standard of T lymphocyte subsets and the red cell immune function.Conclusions The apoptosis rate of CD4 + T lympho,cytes in patients with tuberculosis were significantly higher than the healthy,which led to reducing the number of CD4 + T lymphocytes.There was positive correlation between red cell immunity and T-lymphocyte immunity,and the immunity in red cell and T- lymphocyte was lower than normal controls,which may be related to the immune pathogenesis of pulmonary tuberculosis.
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