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经典型霍奇金淋巴瘤EB病毒感染和p16蛋白表达的相关性研究

The relationship of Epstein-Barr virus infection and expression of p16 protein in classic Hodgkin's lymphoma

摘要:

目的 探讨EB病毒在经典型霍奇金淋巴瘤(CHL)中的感染和p16蛋白表达的关系.方法 应用EBER-1寡核苷酸探针以原位杂交方法检测80例CHL组织中的EBV编码的小RNA;用免疫组化方法检测p16蛋白表达.结果 80例CHL患者中,男52例,女28例,男女之比为1.86:1,其中52例CHL男性患者中,EBER-1阳性率63.46%;28例CHL女性患者中,EBER-1阳性率39.29%,两者比较具有统计学意义(x2=4.298,P=0.038).儿童和老年人中CHL患者共45例,其EBV表达阳性率75.56%,多于成年人的28.57%,两者比较差异具有统计学意义(x2=20.13,P=0.000).EBER-1阳性表达定位在R-S细胞核上,其阳性表达率为55.00%,各亚型CHL的EBV潜伏感染检出率分别是:混合细胞型( MC)71.79%,淋巴消减型(LD) 42.85%,淋巴细胞为主型(LP)47.06%,结节硬化型( NS)29.41%,且MC型明显高于NS型(x2=8.787,P=0.003);p16蛋白阳性表达定位在R-S细胞的细胞核和细胞浆,其总的阳性表达率为45%,各亚型阳性率分别为:LP 47.05%,MC 48.71%,NS35.29%,LD 42.85%;p16蛋白与EBER-1之间呈负相关(r=-0.242,P<0.05).结论 CHL发生可能与EBV感染有关,且p16蛋白的高表达为协助诊断CHL提供了依据.

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abstracts:

Objective To study the relationship between EBV infection of classic Hodgkin's lyphoma and expression of p16 protein.Methods EBER-1 and p16 protein expression in 80 cases of CHL were studied with EBER-1 oligonucleotide probe and immunohistochemistry respectively.Results 80 cases of CHL were enrolled in this study,including male 52 cases,28 cases of female,male to female ratio of 1.86 ∶ 1.The positive rate of EBER-1 in female was 39.29% and in male was 63.46%,which have statistically significant difference (x2 =4.298,P =0.038).The positive rate of EBER-1 in children and the older was significantly higher than that in adult (x2 =20.13,P =0.000).EBER-1 positive expression located in the R-S nuclei which positive expression rate was 55.00%,including mixed cell type (MC) 71.79%,lymphocyte depletion type (LD) 42.85%.lymphocyte predominance (LP) 47.06% and nodular sclerosis type (NS) 29.41%.MG type was significantly higher than NS ( x2 =8.787,P =0.003 ) ; p16 was noted in thc nucleus and cytoplasm.The total positive expression rate was 45%,and subtype positive rates were 47.05% (LP),48.71% ( MC),35.29% (NS) and 42.85% (LD).There was a negative correlation between p16 protein and EBER-1 ( r =-0.242,P < 0.05 ).Conclusions CHL may be related to EBV latent infection and it can be considered as potential markers to identify HRS cells in diagnosis.

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