自分泌运动因子受体的表达与甲状腺乳头状癌的临床关系研究
The relationships between the expression of autocrine motility factor receptor and the clinical features of papillary thyroid carcinoma
目的 探讨自分泌运动因子受体(autocrine motility factor receptor,AMFR)在甲状腺乳头状癌中的表达情况,以及其与临床的关系.方法 采用实时荧光定量PCR和免疫组化的方法对甲状腺乳头状癌进行研究.结果 甲状腺乳头状癌和甲状腺正常组织在AMFR的mRNA和蛋白水平差异均有统计学意义(6.296±1.568 vs7.913±2.351,t=3.681,P=0.001;63.1%vs 34.5%,x2 =13.722,P<0.001).免疫组化分析显示甲状腺乳头状癌中AMFR蛋白的水平与肿瘤大小(x2=5.209,P<0.05)和淋巴结转移有关(x2=4.32,P<0.05),而与性别(x2=0.739,P=0.39)、年龄无关(x2 =0.064,P=0.81).结论 AMFR在甲状腺乳头状癌中表达升高,其可能成为甲状腺乳头状癌治疗中的一个新靶点和预后的新指标.
更多Objective To investigate the expression of autocrine motility factor receptor (AMFR)in papillary thyroid carcinoma and its relationship with clinical characteristics of this disease.Methods Real - time quantitative PCR and immunohistochemical methods were used to analyze the expression of AMFR in papillary thyroid carcinomas.Results The significant differences in AMFR expression between papillary thyroid carcinoma and normal thyroid tissues were found in the levels of mRNA (6.296 ± 1.568 vs 7.913 ± 2.351,t=3.681,P=0.001 ) and protein ( 63.1% vs 34.5 %,x2=13.722,P < 0.001 ),respectively.Immunohistochemistry analyses showed that the protein expression of AMFR in papillary thyroid carcinomas were significantly correlated with tumour size ( x2=5.209,P < 0.05 ) and lymph node metastasis ( x2=4.32,P < 0.05 ),and it was affected by the factors age ( x2=0.739,P=0.39 ) and gender ( x2=0.064,P=0.81 ).Conclusions The increased AMFR in papillary thyroid carcinoma would be a new target for cancer therapy and a new marker for prognosis.
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