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目的 检测结直肠癌组织中T淋巴瘤侵袭转移诱导因子1(Tiam1) mRNA表达量的变化,探讨其与结直肠癌侵袭转移及预后的关系.方法 以实时荧光定量RT-PCR检测97例结直肠癌组织、49例结直肠腺瘤息肉及30例正常结直肠黏膜组织中Tiam1 mRNA的表达量;分析癌组织中Tiam1 mRNA表达量与结直肠癌侵袭转移及预后的关系.结果 结直肠癌组Tiam1 mRNA表达量显著高于结直肠腺瘤息肉组和正常对照组[(14.45 ±4.87)2-△△Ct vs (3.77 ±3.15)2-△△Ct,(1.06±0.65)2-△△Ct,P＜0.01];腺瘤息肉中伴高级别上皮内瘤变者Tiam1 mRNA表达量明显高于伴低级别上皮内瘤变者[(5.26±2.45)2-△△Ctvs (3.78 ±2.19)2-△△Ct,P＜0.01],两者均明显高于不伴上皮内瘤变者[(2.13±1.64)2-△△t,P ＜0.05或P＜0.01].结直肠癌组Tiam1 mRNA表达量与肿瘤分化程度、浸润深度、淋巴结转移及TNM分期呈正相关(P＜0.01),与术后复发呈正相关(P＜0.01),与术后生存期呈负相关(P＜0.01).Cox多因素分析显示癌组织中Tiam1 mRNA表达量是影响结直肠癌术后预后的高危因素(Exp=4.043).结论 癌组织中Tiam1 mRNA表达升高与结直肠癌侵袭转移及不良预后密切相关,可作为评价其预后的指标;结直肠腺瘤息肉组织中Tiam1 mRNA高表达可能与结直肠癌发生相关.

Objective To investigate the relationship between quantitative detection of T lymphoma invasion and metastasis inducing factor (Tiaml) mRNA and invasion,metastasis and prognosis of patients with colorectal cancer.Methods The quantitative expression of Tiam1 mRNA in cancer tissues in colorectal cancers (n =97),in tissues of colorectal adenomatous polyposis (n =49) and normal control (n =30) were detected with real-time fluorescence quantitative-polymerase chain reaction (PCR).The relationship between the quantitative expression of Tiam1 mRNA in colorectal cancer tissues and the invasion,metastasis and the prognosis were analyzed.Results The levels of Tiam1 mRNA expression in the colorectal cancer group,were significantly higher than those in colorectal adenomatous polyposis group and normal controlgroup [(14.45±4.87)2-△△△Ct vs (3.77 ±3.15)2-△△Ct,(1.06 ±0.65)2-△△Ct,P ＜0.01].The levels of Tiam1 mRNA expression in the adenomatous polyposis with high grade intraepithelial neoplasia were signifiCantly higher than those in the adenomatous polyposis with low grade intraepithelial neoplasia [(5.26 ±2.45)2-△△Ct vs (3.78 ±2.19)2-△△Ct,P ＜0.01],which were both significantly higher than those in the adenomatous polyposis with no intraepithelial neoplasia [(2.13 ± 1.64)2-△△Ct,P ＜0.05 or P ＜ 0.01].The levels of Tiam1 mRNA expression in colorectal cancer group were positively related to the differentiated degree of tumor,depth of invasion,lymph node metastasis,tumor node metastasis (TNM) stages and the postoperative recurrence (P ＜ 0.01),and were negatively related to the postoperative survival time (P ＜0.01).Multivariate Cox regression analysis revealed that levels of Tiam1 mRNA expression in cancer tissues were independent prognostic factor for colorectal cancer (Exp =4.043).Conclusions The high Tiam1 mRNA expression levels in cancer tissues are closely related to invasion,metastasis and poor prognosis of colorectal cancer,and may be a prognostic indicator of colorectal cancer.The increase expression of Tiam1 mRNA in colorectal adenomatous polyposis may be related to colorectal tumorigenesis.