miR-346在结肠癌细胞中的表达及功能研究
Expression and function of miR-346 in colon cancer cells
目的 检测结肠癌细胞中miR-346表达及侵袭转移的关系,为结肠癌预测及治疗靶点提供试验依据.方法 采用qRT-PCR法(quantificational real-time polymerase chain reaction)检测结肠癌细胞株(HCT116、COLO205、SW620)及正常结肠上皮细胞(NCM460)中 miR-346 的表达.采用miR-346拟似剂( mimic)或抑制剂( inhibitor)对miR-346表达进行上调或下调并转染HCT116细胞, qRT-PCR检测转染效率,Transwell观察转染后HCT116细胞转移及浸润行为变化.结果 miR-346在结肠癌细胞株HCT116、COLO205、SW620中相对表达量分别为0. 372±0. 068、1. 284±0. 253、1. 105 ±0. 203,在正常结肠上皮细胞( NCM460)中相对表达量为 0. 126 ± 0. 004,差异有统计学意义( F 值=4. 563,P<0. 05),与HCT116组比较,COLO205组及SW620组差异有统计学意义(t值分别为7. 256、5. 419,P均<0. 01).与 NCM460 组比较,COLO205 组及 SW620 组差异有统计学意义(t 值分别为9. 652、7. 753,P均<0. 01). miR-346 表达上调后 HCT116 细胞侵袭及转移能力增强( t 值分别为2. 458、2. 194,P 均<0. 05),miR-346 表达下调后 HCT116 细胞侵袭及转移能力减弱(t 值分别为2. 247、2. 065,P均<0. 05).结论 miR-346在结肠癌细胞中表达高,调控结肠癌细胞侵袭及转移.
更多Objective To detect the expression of miR-346 in colon cancer cells and the relationship with invasion and metastasis, so as to provide experimental basis for predicting and treating targets of colon cancer. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of microRNA-346 in colon cancer cell lines ( HCT116, COLO205, SW620) and normal colon epithelial cells ( NCM460 ) . The expression of microRNA-346 was up-regulated or down-regulated by mimic or inhibitor and transfected into HCT116 cells. The transfection efficiency was detected by qRT-PCR. The metastasis and infiltration behavior of HCT116 cells were observed by Transwell. Results The relative expression of microRNA-346 in colon cancer cell lines HCT116, COLO205 and SW620 were (0. 372±0. 068),(1. 284±0. 253),(1. 105±0. 203),respectively. The relative expression of COLO-205 and SW620 in normal colon epithelial cells (NCM460) was (0. 126±0. 004). Compared with NCM460 group, COLO-205 group and SW620 group had significant difference (t=9. 652,P<0. 01; t=7. 753,P<0. 01). The invasion and metastasis ability of HCT116 cells increased after the up-regulation of the expression of microRNA-346 (t=2. 458,P<0. 05; t=2. 194,P<0. 05),and decreased after the down-regulation of the expression of microRNA-346 (t=2. 247,P<0. 05; t=2. 065,P<0. 05) . Conclusion Mir-346 is highly expressed in colon cancer cells and regulates invasion and metastasis of colon cancer cells.
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