摘要目的 对甲状腺乳头状癌(PTC)的蛋白质组进行研究,并与正常组织对比,寻找其中差异显著的蛋白.方法 应用蛋白质组学技术,提取10例PTC与周围正常甲状腺组织(NT)的蛋白质样品进行双向电泳分离及对比,应用Image Master软件对电泳图谱进行分析;将选出的差异性蛋白质点进行质谱鉴定和数据库查询.结果 得到了PTC及NT的双向电泳图谱;通过软件分析、对比,从中初步选取4个差异蛋白质点进行质谱鉴定,确认为4种相应的蛋白质:其中脂皮质蛋白(annexinⅠ)、peroxiredoxin Ⅰ、线粒体顺乌头酸酶(mitochondrial aconitase)在PTC中表达上升,碳酸酐酶Ⅰ(carbonic anhydrase Ⅰ)在PTC中表达下降.结论 蛋白质组学为PTC的研究提供了新的发展方向,在寻找肿瘤的特异性蛋白标记物、进一步探究肿瘤的发病机制以及寻找治疗肿瘤的分子靶点等方面发挥作用.

abstractsObjective To study the proteomics of papillary thyroid carcinoma (PTC), and to detectthe differential expression of proteins compared to normal thyroid (NT) tissue. Methods Protein extracts ofpapillary thyroid carcinoma and normal thyroid tissues from 10 patients were separated by proteomicstechnique and identified using Image Master software. The selected differential proteins were sent toProteomics Research Center of Chinese Academy of Medical Sciences for mass-spectrometry identification.Results The basic 2-dimensional electrophoresis (2-DE) images of PTC and NT were obtained, and fourpotential differential expression spots were selected for mass-spectrometry identification. Among them,annexin Ⅰ , peroxiredoxin Ⅰ , mitochondrial aconitase were up-regulated while carbonic anhydrase Ⅰ wasdown-regulated. Conclusion Proteomics may provide a new direction to study PTC, by identifying sometumor-specific markers, leading to elucidation of the mechanism of tumorigenesis and identification oftherapeutic targets.