摘要目的 评价乳腺癌七皮间质转化(EMT)与基底细胞样型乳腺癌(BLBC)的关系.方法 依据免疫表型从458例浸润性乳腺癌中筛选出基底细胞样型、管腔A型、管腔B型和HER2过表达型共382例.免疫组织化学EnVision二步法检测FOXC-2、波形蛋白、Syndecan-1和E-cadherin在乳腺癌中表达,并分析其与BLBC的关系.结果 41例BLBC中癌细胞FOXC-2、波形蛋白和Syndecan-1的阳性率以及E-cadherin表达减弱率分别为34.1%(14/41)、43.9%(18/41)、87.7%(36/41)和63.4%(26/41).BLBC的FOXC-2和波形蛋白阳性率均高于其他亚型乳腺癌,其E-cadherin表达较其他亚型乳腺癌下降(P<0.01).BLBC肿瘤间质细胞Syndecan-1阳性率为41.5%(17/41),高于其他亚型乳腺癌(P=0.007).癌细胞FOXC-2表达与波形蛋白表达呈正相关(r=0.607,P<0.01).FOXC-2和波形蛋白阳性组腋窝淋巴结转移率分别为71.4%和66.7%,分别高于阴性组(P=0.002和P=0.001).结论 本结果显示乳腺癌中EMT与BLBC关系最密切.EMT可能是造成BLBC与其他亚型乳腺癌生物学行为差异的蕈要原因.

abstractsObjectives To evaluate the relationship between epithelial- mesenchymal transition and basal cell-like phenotype breast cancer(BLBC). Methods Three hundred and eighty two cases of breast cancers including basal cell-like, luminal A, luminal B and Her-2 subtypes were collected from 458 cases of invasivc breast cancers based on their immunopbenotypos. They were then stained immunohistochemicallywith FOXC-2,vimentin,Syndecan-1 and E-cadherin. The relationship of these markers with the basal cell-like phenotype of breast cancer was studied. Results Of the 41 BLBC cases, FOXC-2, vimentin and Syndecan-1 were positive in 14 cases (34. 1% ), 18 cases (43.9%) and 36 cases (87.7%) respectively;E-cadherin expression was reduced in 26 cases (63.4%). The positive rates of FOXC-2 and vimentin were higher in BLBC than in other subtypes of breast cancer (P < 0. 01 ). The expression of E-cadherin was the lowest among the 4 subtypes of breast cancers ( P < 0. 01 ). Syndecan-1 was positive in the stromal cells adjacent to cancer cells in 17 cases (41.5%) BLBC and the expression was higher than that in other subtypes (P = 0. 007 ). There existed a correlation between FOXC-2 and vimenfin expression in BLBC (r =0. 607, P < 0. 01 ). The rates of positive lymph nodes in FOXC-2 and vimentin positive BLBC cases were 71.4% and 66. 7% respectively, and beth were higher than those of FOXC-2 and vimenfin negative BLBC cases ( P = 0. 002 and P = 0. 001 ). Conclusion Epithelial-mesenchymal transition is probably related to the basal cell-like phenotype of breast cancers, and this may be one of the reasons accounting for the different biological behavior of BLBC from other subtypes of breast cancer.