摘要目的 探讨CD147和基质金属蛋白酶(MMP)-2和MMP-9与转化生长因子(TGF)β1及其Ⅰ型受体(TGFβR I)在乳腺癌组织中的表达与肿瘤转移和预后及其相互间的关系.方法 建立组织芯片平台,应用免疫组织化学SP法检测160例乳腺癌组织CD147、MMP-2、MMP-9、TGFβ1和TGFβRI蛋白的表达情况,并进行了8～64个月随访.结果 160例乳腺癌中CD147、MMP-2、MMP-9、TGFβ1和TGFβRI阳性率分别为87.5%(140例)、96.9%(155例)、95.0%(152例)、73.7%(118例)和60.6%(97例);CD147的表达与乳腺癌腋淋巴结受累、TNM分期和HER2过表达均呈正相关(P<0.01,P<0.05和P<0.01),与孕激素受体表达呈负相关(P<0.05);CD147蛋白阳性患者(55.9个月)无复发生存期显著低于阴性患者(46.1个月,P=0.023);CD147与MMP-2、MMP-9蛋白的表达均呈显著正相关(r=0.728和r=0.399,均P<0.01);CD147蛋白表达与TGFβ1和TGFβRⅠ表达均呈显著正相关(r=0.223,P<0.01和r=0.191,P<0.05).结论 乳腺癌组织中CD147表达状况与肿瘤侵袭转移和患者预后有密切关系;CD147的表达与MMP-2、MMP-9、TGFβⅠ和TGFβR Ⅰ表达状况关系密切.

abstractsObjective To investigate the expression of CD147, matrix metalloproteinase (MMP)-2, MMP-9, Transforming growth factor (TGFβ1) and TGFβR Ⅰ proteins and their relationships to breast cancer. Methods The expression of CD147, MMP-2, MMP-9, TGFβ and TGFβR Ⅰ proteins was examined on tissue chips containing 160 cases of breast carcinomas by S-P immunohistoehemieal method.Results The positive rates of CD147, MMP-2, MMP-9, TGFβ1 and TGFβR Ⅰ proteins were 87.5% (140/160), 96.9% ( 155/160), 95.0% ( 152/160), 73.7% (118/160) and 60.6% (97/160),respectively. The expression of CD147 was positively correlated with axillary lymph node metastasis, TNM staging and HER2 over expression ( P < 0. 01, P < 0. 05 and P < 0. 01, respectively ), and inversely correlated with PR expression ( P < 0.05 ). The patients' relapse-free survival was shorter in TGFβ1-positive group than in TGFβ1 negative group (P <0. 05). Both the expression of MMP-2 and MMP-9 were positively correlated with CD147 expression; and both the expression of TGFβ1 and TGFβR Ⅰ were positivelycorrelated with CD147 expression (P <0. 01 and P <0. 05, respectively). Conclusion The expression of CD147 is considered closely correlated with tumor invasion, metastasis and prognosis in breast cancer, and has also a close correlation with MMP-2, MMP-9, TGFβ1 and TGFβR Ⅰ expression.