摘要目的 初步评价BIOMED-2引物在辅助诊断眼附属器淋巴瘤的应用价值.方法 收集63例眼附属器淋巴瘤,均为甲醛固定的石蜡包埋标本,提取基因组DNA并通过扩增管家基因β-actin检测其质量,应用BIOMED-2标准化基因重排检测系统中IgH_B和IgK_B两套多重PCR引物进行Ig基因的PCR扩增,并利用基因扫描技术对扩增产物进行克隆性分析.结果 76.2%(48/63)淋巴瘤石蜡包埋标本的DNA可扩增出300 bp大小的β-actin,适于基因重排检测.IgH_B和IgK_B多重PCR引物的淋巴瘤检出率分别为79.2%(38/48)和68.8%(33/48),二者联合的检出率为91.7%(44/48).结论 应用较少的BIOMED-2引物结合基因扫描技术能检测出大多数眼附属器淋巴瘤,对临床病理诊断具有较高的辅助价值.

abstractsObjective To assess the practical value of BIOMED-2 primers in the diagnosis of ocular adnexal lymphoma by PCR. Methods DNA was extracted from 63 formalin-fixed paraffin-embedded (FFPE) ocular adnexal lymphoma specimens. The DNA quality was evaluated by PCR-based amplification of housekeeping gene β-actin. IgH_B and IgK_B primers of BIOMED-2 standardized clonality analysis system were used to evaluate the immunoglobin gene rearrangements. PCR products were analyzed using capillary electrophoresis and GeneScan software. Results 76.2% (48/63) of FFPE samples produced amplifiable DNA for detection of Ig gene rearrangements. Positive detection rates by BIOMED-2 IgH_B and IgK_B primers were 79. 2% (38/48) and 68. 8% (33/48) , respectively, with a combined positive detection rate of 91. 7% (44/48). Conclusions IgH_B and IgK_R primers of BIOMED-2 are suitable for the detection of clonal rearrangements of Ig gene using FFPE specimens of ocular adnexal lymphomas.