摘要目的 研究维吾尔族妇女宫颈癌中人乳头状瘤病毒(HPV)感染和人类白细胞抗原Ⅰ类(HLA-Ⅰ)家族基因HLA-A、B和C表达的关系,探讨HPV感染和HLA-Ⅰ类家族基因表达缺失在宫颈癌演进过程中的作用.方法 收集维吾尔族妇女宫颈炎、宫颈上皮内瘤样病变(CIN)Ⅰ/Ⅱ/Ⅲ和宫颈鳞癌患者的新鲜组织标本共78例,提取总RNA,采用半定量RT-PCR方法鉴定HLA-A、B和C基因的mRNA表达水平.提取组织DNA,采用HPV通用引物和HPV分型芯片确定HPV亚型.结果 HLA-A、B和C基因的总体mRNA表达缺失率随着宫颈病变的加重而增加,在宫颈炎组织内为1/12,在CIN及宫颈鳞癌分别占70.0%(14/20)和84.8%(39/46),在恶性程度高的低分化癌组织中高达90.6%(29/32),并与高危型HPV16感染呈正相关(r=0.803,P＜0.01).结论 HLA-Ⅰ类基因的表达缺失是维吾尔族妇女宫颈癌发生的重要标志,而HPV16感染可能是HLA-Ⅰ分子表达缺失的前提条件.

abstractsObjective To explore the relationship between human papillomavirus(HPV)infection and expression of human leukocyte antigen class Ⅰ(HLA-Ⅰ)family genes(HLA-A,B and C)in cervical cancers of Uighur women,and to investigate their effect on cervical cancer progression.Methods Fresh tissue samples of 78 Uighur women with cervical aquamous carcinoma,cervical intraepithelial neoplasia (CIN)or benign cervicitis were selected.HLA-A,B and C expression and HPV infection were analyzed using RT-PCR and HPV gene chips,respectively.Results There was a tendency of increasing the total less of HLA-A,B and C mRNA as the cervical lesions became more aggressive.Loss of HLA-Ⅰ mRNA in CIN (Ⅰ,Ⅱ and Ⅲ)and cervical squamous carcinoma was 70.0%(14/20)and 84.8%(39/46)respectively.Poorly differentiated cervical carcinomas had the highest HLA-Ⅰ expression loss(90.6%).In contrast,HLA-Ⅰ mRNA loss was seen in only 8%of cases of cervicitis.Moreover,it was found that high risk HPV 16 infection was strongly correlated with the loss HLA-Ⅰ mRNA expression(r=0.803,P＜0.01).Conclusions The loss of HLA-Ⅰ gene expression is strongly correlated with HPV-16 infection,and may serve as a biomarker of cervical cancer progression in Uighur women.