摘要目的 探讨胰腺癌的细胞遗传学特征.方法 采用光谱核型分析技术对中国人胰腺癌细胞系P2的染色体核型进行分析,并选择EGFR/CEP 7双色荧光原位杂交(FISH)探针,对比分析10例胰腺癌和10例慢性胰腺炎石蜡标本的EGFR基因拷贝数,验证光谱核型分析结果.结果 P2细胞系为亚三倍体核型,共发现26种染色体异常,其中重复出现的染色体异常改变为染色体4、9、18、19、22、Y、10p、15p、8p、6q和12p缺失,染色体7和12q增加,以及染色体结构畸变der(9;15)(q10;q10)、der(10)(3;10)(?;q26)和der(12)(8;12)(?;p13).EGFR-FISH阳性为4/10.结论 胰腺癌细胞系的染色体重排非常复杂,进一步扩大样本量进行相关分析,包括了解胰腺癌的EGFR-FISH阳性率非常有必要.

abstractsObjective To investigate the chromosomal characteristics of pancreatic ductal adenocarcinomas by spectral karyotyping. Methods Cytogenetic aberrations of pancreatic cancer cell line P2 established from a Chinese patient was investigated by spectral karyotyping (SKY). Chromosomal alterations were further evaluated in 10 cases of pancreatic cancer and 10 cases of chronic pancreatitis by two color fluorescence in situ hybridization (FISH) by using EGFR/CEP7 probe and paraffin embedded tissue samples. Results Hypotriploid and 26 chromosomal aberrations were revealed in cell line P2. Recurrent chromosomal numerical alterations included loss of chromosome 4,9, 18,19,22, Y, 10p, 15p, 8p, 6q and 12p, with gain of chromosome 7 and 12q. Frequent chromosomal structural abnormalities included der (9;15) (q10;q10), der(10) (3;10) (?;q26) and der(12) (8;12) (?;p13). Four of 10 cases showed EGFR copy number changes by FISH. Conclusions Highly complex chromosomal rearrangements occur in pancreatic cancers. Additional studies of more cases are needed, including FISH analysis of EGFR copy number changes, to reach a conclusion.