摘要目的 探讨乳腺浸润性微乳头状癌(IMPC)中前列腺干细胞抗原(PSCA)在蛋白和mRNA水平的表达及其与临床病理特征的关系.方法 采用免疫组织化学(LSAB法)检测66例IMPC、67例非特殊型浸润性导管癌(IDC-NOS)中PSCA的表达,比较其差异并分析PSCA的表达与IMPC临床病理特征之间的关系.采用逆转录聚合酶链反应(RT-PCR)检测10例IMPC和10例IDC-NOS癌组织及其对应的正常乳腺组织中PSCA mRNA的表达水平.结果 免疫组织化学染色结果显示,PSCA在66例IMPC中有47例高表达(71.2%,47/66),67例IDC-NOS中35例高表达(52.2%,35/67),两组表达水平之间的差异具有统计学意义(P=0.024);IMPC中PSCA的表达与淋巴结转移呈正相关(P=0.039).RT-PCR结果显示,PSCA在10例IMPC和10例IDC-NOS癌组织中各有7例和5例表达,且全部高于对应的正常乳腺组织;IMPC癌组织中PSCA表达强度明显高于IDC-NOS癌组织.结论 PSCA的表达可能在IMPC淋巴结转移过程中发挥了重要作用.

abstractsObjective To study the expression of prostate stem cell antigen (PSCA) at protein and mRNA levels in invasive micropapillary carcinoma of the breast (IMPC) and to analyze the relationship between PSCA expression and clinicopathologic features. Methods The expression of PSCA protein was analyzed by immunohistochemistry (LSAB) in 66 cases of IMPC and 67 cases of invasive ductal carcinoma, not otherwise specified (IDC-NOS). The association between PSCA expression and clinicopathologic features was also analyzed in IMPC. Furthermore, RT-PCR was used to detect PSCA mRNA in 10 cases of primary IMPC and 10 cases of primary IDC-NOS with paired normal breast tissues, each from the same subject. Results Immunohistochemical analysis revealed the overexpression of PSCA in 47 of 66 (71.2%) cases of IMPC and 35 of 67 (52.2%) IDC-NOS. Statistical analysis showed a significant difference of PSCA expression between IMPC and IDC-NOS (P=0.024). In IMPC, the expression of PSCA was correlated with lymph nodes metastasis (P=0.039). RT-PCR showed the mRNA level of PSCA was significantly higher in primary IMPC and IDC-NOS tissue than that in paired normal breast tissue (7/10 and 5/10, respectively), and it was also significantly higher in primary IMPC tissue than that in IDC-NOS tissue.Conclusion PSCA might play an important role in lymph node metastasis in IMPC.