摘要目的 探讨神经源性分化蛋白(NeuroD)在胰腺外分泌癌中的表达及其意义.方法 应用组织芯片和免疫组织化学EnVision二步法研究NeuroD、增殖细胞核抗原(PCNA)、p53在127例胰腺外分泌癌中的表达情况,并在光镜下观察胰腺癌神经组织浸润、神经周围淋巴细胞套、癌旁慢性胰腺炎、胰周淋巴结转移情况.分析NeuroD的表达与上述其他指标及性别、年龄、肿瘤部位、肿瘤组织学类型及分化程度等的关系.结果 NeuroD、PCNA和p53蛋白在胰腺癌组织中的阳性率分别为64.6％(82/127)、57.5％ (73/127)和59.1％ (75/127),与菲癌组织[分别为10.5％ (8/76)、9.2％(7/76)和9.2％(7/76)]相比,差异有统计学意义(P＜0.01).NeuroD蛋白的表达与PCNA、p53蛋白的表达和胰腺癌肿瘤神经浸润之间有统计学相关性(P＜0.05),而与性别、年龄、肿瘤部位、肿瘤组织学类型及分化程度、癌旁慢性胰腺炎、神经周围淋巴细胞套和淋巴结转移均等无统计学相关性(P＞0.05).结论 NeuroD蛋白在胰腺癌中呈高表达,可能参与了胰腺癌的发生和进展,并且与胰腺癌的增殖、p53信号通路和肿瘤神经浸润之间密切相关.

abstractsObjective To investigate the expression and significance of neurogenic differentiation protein (NeurodD) in pancreatic carcinoma.Methods The expression of NeuroD,PCNA and p53 proteins in 127 specimens of pancreatic carcinoma was detected by tissue microarray and immunohistochemestry.The correlations were analyzed between NeuroD and PCNA.p53,neural invasion,sleeve-like lymphocytic infiltration around the nerve,pancreatitis odjacent to carcinoma,lymph node metastasis and age,gender,location of tumors,histological types and differentiation of pancreatic carcinomas.Results The positive rates of NeuroD,PCNA and p53 expression were higher in pancreatic carcinoma than those in non-tumor pancreatic tissues [64.6％ (82/127)vs 10.5％ (8/76),57.5％ (73/127)vs9.2％ (7/76),59.1％(75/127) vs 9.2％ (7/76),P ＜ 0.01 ].NeuroD expression in pancreatic carcinoma was related to that of PCNA and p53 and neural invasion ( P ＜ 0.05 ).No significant correlation was found between NeuroD and age,gender,tumor location,histological types and differentiation,sleeve-like lymphocytic infiltration,pancreatitis adjacent to the carcinoma and lymph node metastasis in pancreatic carcinomas.Conclusions NeuroD overexpression in pancreatic (e)arciuoma.The overexpression of NeuroD may contribute to the tumorogenesis and development of pancreatic carcinoma,and is closely correlated to the cancer cell proliferation,p53 signal pathway and neural invasion in pancreatic carcinoma.