摘要目的 探讨间质细胞telocytes在小鼠气管和肺组织中的分布特征.方法 应用电镜、免疫组织化学、原代细胞培养及活细胞染色方法确定间质细胞telocytes在小鼠气管和肺组织中的分布特征.结果 扫描及透射电镜均在小鼠气管软骨、平滑肌之间的间隙中找到了telocytes；在小鼠肺组织中,telocytes位于肺间质,紧邻毛细血管及小支气管.免疫组织化学检测发现telocytes表达c-kit、波形蛋白、CD34.从肺组织中分离培养得到的细胞具有典型的telocytes特征,即带有telopode的细胞,telopode也就是telocytes细胞胞体的延伸部分.透射电镜显示telopode呈管腔样结构,且厚薄交替,厚的部分称为podom,薄的部分称为podomer.结论 telocytes位于气管环状软骨和平滑肌之间的间隙中；在肺组织主要分布于小静脉、小支气管的间质中.

abstractsObjective To explore the location of telocytes in pulmonary tissues. Methods Electron microscopy,immunohistochemistry,primary cell culture and vital cell staining were used to identify the distribution of telocytes in mouse bronchial and pulmonary tissues.Results Telocytes were identified in the interstitial space between bronchial cartilage (cricoid) and smooth muscle by scanning and transmission electron microscope in mouse.By transmission electron micrscope and immunohistochemistry,telocytes were found in the interstitial spaces of lung parenchyma in connection with capillaries and bronchia.Telocytes expressed CD34, c-kit and vimetin by immunohistochemistry. After isolation, cultured telocytes demonstrated typical morphological feature,i.e.cells with telopode,which was seen as luminal structures with alternating thin and thick segments under electron microscope.Conclusion Telocytes are present in the interstitial space between cricoid cartilage and smooth muscle.