摘要目的 通过研究Runx3基因CpG岛甲基化对胃癌病变的影响,探讨甲基化特异性聚合酶链反应(MSP)法与焦磷酸测序(PS)法对Runx3基因甲基化检测的特异性.方法 选取150例胃癌患者胃切除标本及50例无癌变胃黏膜标本,以MSP法和PS法同时检测其Runx3启动子的甲基化程度.结果 与正常胃黏膜相比,胃癌组织Runx3的甲基化程度差异有显著性意义(MSP法:67.3％比40.0％,P=0.002；PS法:76.0％比30.0％,P＜0.01).MSP法显示Runx3甲基化只与肿瘤大小相关(P＜0.05).而PS法分析显示进展期胃癌的Runx3甲基化率高于早期胃癌(P＜0.05)；且其甲基化状态与肿瘤的大小(P =0.004)、Lauren分型(P=0.043)、浸润的深度(P＜0.01)、淋巴结转移(P=0.021)及肿瘤TNM临床分期(P=0.045)等临床病理特征之间存在相关性.结论 Runx3启动子甲基化与胃癌临床病理特征包括肿瘤大小、Lauren分型、浸润的深度、淋巴结转移以及肿瘤TNM临床分期密切相关；与MSP法相比,PS法敏感性更高,且检测结果与临床病理学结果之间有显著相关性,对于胃癌的早期诊断和治疗有着重要意义.

abstractsObjective To investigate the role of Runx3 gene CpG island methylation in the development of human gastric carcinoma.Methods A total of 150 tumor specimens from patients with gastric carcinoma and 50 normal tissue specimens were selected. Methylation specific PCR (MSP) and pyrosequencing (PS) were used to detect the methylation status of Runx3 gene promoter. Results Compared to normal tissue samples,a significant increase of CpG island methylation status of Runx3 gene was observed in gastric carcinomas ( MSP:67.3％ vs.40.0％,P =0.002 ; PS:76.0％ vs.30.0％,P ＜0.01).Runx3 gene methylation was only related to tumor size ( P ＜ 0.05 ) based on MSP analysis.PS test however showed that the extent of methylation of Runx3 gene was related to the tumor size ( P =0.004 ),Lauren's classification ( P =0.043 ),depth of invasion ( P ＜ 0.01),lymph node metastasis ( P =0.021)and TNM staging ( P =0.045 ).Conclusions Methylation status of Runx3 gene detectable by PS is closely correlated with clinicopathological parameters of gastric carcinoma, including tumor size, Lauren' s classification,depth of invasion,lymph node metastasis and TNM staging.PS is more sensitive than MSP in the detection of Runx3 gene methylation,which may serve as an important marker for early diagnosis and treatment of gastric carcinoma.