摘要目的 探讨RNA干扰Ezrin对胰腺癌胞系Panc-1细胞生物学行为的影响.方法 建立靶向Ezrin干扰质粒,稳定转染Panc-1细胞,并用流式细胞术检测细胞周期,CCK-8法检测细胞增殖情况,扫描电镜观察细胞表面形态及表面突起的变化,软琼脂克隆形成实验检测细胞体外非锚着依赖性的生长能力,用未包被及包被Matrigel胶的Transwell小室分别检测细胞的运动和侵袭能力.结果 RNA干扰Ezrin后其蛋白的表达明显下降,Panc-1表面的细胞突起、微绒毛数量明显减少,体外非锚着依赖性的生长能力也明显下降,运动及侵袭能力也明显下降,但对细胞的体外增殖和细胞周期没有明显的影响.结论 Ezrin蛋白的表达对胰腺癌细胞的细胞突起、表面微绒毛的形成、细胞骨架及非锚着依赖性的生长发挥着重要的作用,干扰Ezrin的表达将对胰腺癌细胞的表面突起的形成、细胞骨架以至细胞运动及在转移灶的存活中起着重要的作用.

abstractsObjective To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.Methods Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid.The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis,respectively.Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy.Colony formation assay was used to determine the cell anchor-independent growth ability in vitro.Trans-filter migration and invasion assays were performed with 8 μm pore inserts in a 24-well BioCoat chamber with/without Matrigel.Results Ezrin silencing decreased cellular protrusions/microvilli formation,anchorage-independent growth,cell migration and invasion,but had no effects on cell proliferation in vitro and cell cycle,in pancreatic cancer cell line Panc-1.Conclusion Ezrin expression affects the cellular protrusions/microvilli formation,anchorage-independent growth,cell migration and invasion in pancreatic cancer cell line Panc-1.