摘要目的 检测星形胶质细胞上调基因1(AEG-1)在喉鳞状细胞癌(鳞癌)组织中的表达,并探讨其表达与喉鳞癌临床病理特征和预后之间的关系.方法 逆转录聚合酶链反应(RT-PCR)测定AEG-1 mRNA在13对配对喉鳞癌及癌旁黏膜组织中的表达.同时,免疫组织化学技术检测AEG-1蛋白在88例喉鳞癌及15例癌旁黏膜组织中的表达,并统计分析其表达水平与临床病理特征及预后的关系.结果 AEG-I蛋白在15例癌旁黏膜组织中仅呈阴性或弱阳性表达；AEG-1 mRNA在喉鳞癌组织中的相对表达量较癌旁黏膜显著升高(0.81 ±0.17比0.23 ±0.10;t=10.337,P＜0.01)；88例喉鳞癌组织中,AEG-1蛋白的阳性表达率为87.5％(77/88).且AEG-1蛋白表达水平与喉鳞癌患者T分期(x2 =6.289,P =0.018)、临床分期(x2=11.049,P＜0.01)、转移(x2=20.859,P＜0.01)及术后复发(x2=13.459,P＜0.01)密切相关.生存分析显示AEG-1蛋白高表达组与低表达组患者5年总生存率分别为35.9％与86.4％,差异具有统计学意义(x2=23.409,P＜0.01)；多因素分析示AEG-1蛋白表达水平为喉鳞癌患者预后的独立影响因素(P=0.016).结论 AEG-1蛋白可能在喉鳞癌的发生、发展中发挥了重要作用,对喉鳞癌患者的预后具有评估价值.

abstractsObjective To assess the protein expression ot astrocyte elevated gene-1 (AEG-1) in tissue specimens of laryngeal squamous cell carcinoma (LSCC),and to correlate its expression with clinicopathological paralneters and prognosis in patients with LSCC.Methods RT-PCR was used to assay the expression of AEG-1 mRNA in 13 pairs of LSCC tissues and their corresponding noncarcinoma epithelia.Immunohistochemistry was performed on paraffin-embedded tissue specimens to investigate the protein expression of AEG-1 in 88 cases of LSCC specimens and 15 cases of adjacent epithelial samples.Results The expression of AEG-1 mRNA was significantly increased in LSCC tissues compared to adjacent noncarcinoma epithelial tissues (0.81 ± 0.17 vs.0.23 ± 0.10; t =10.337,P ＜ 0.001).Meantime,the positive rate of AEG-1 protein in 88 cases of LSCC was 87.5％ (77/88).However,15 cases of adjacent noncarcinoma epithelial merely demonstrated negative or mild expression of AEG-1 protein.AEG-1 overexpression was closely correlated with T stage (x2 =6.289,P =0.018),clinical stage (x2 =11.049,P ＜ 0.01),metastasis (x2 =20.859,P ＜ 0.01) and recurrence (x2 =13.459,P ＜ 0.01).The overall survival rates of patients with AEG-I overexpression and low expression were 35.9％ and 86.4％,respectively (x2 =23.409,P ＜0.01).Multivariate Cox regression analysis revealed that AEG-1 expression was an independent prognostic factor(P =0.016).Conclusion AEG-1 protein may play a critical role in the initiation and progression of LSCC,implicating its predictive value in prognosis.