摘要目的 分析EB病毒感染与维吾尔族霍奇金淋巴瘤(HL)患者临床病理特征的相关性及研究EB病毒阳性的维吾尔族HL患者中潜伏膜蛋白(LMP) 2A蛋白的表达状况.方法 用原位杂交的方法分别对40例维吾尔族HL及20例淋巴结反应性增生组织标本进行EB病毒检测；以免疫组织化学(PV9000通用型法)检测EB病毒阳性的HL患者LMP2A蛋白表达状况.结果 40例维吾尔族HL患者标本中,EB病毒编码的小分子RNA(EBER)阳性率为65.0％ (26/40),淋巴结反应性增生组中EBER阳性率为5/20,两组间差异具有统计学意义(P＜0.05).EBER阳性率在HL患者的不同年龄、性别、分期、类型及是否伴有巨大肿块各组间差异无统计学意义(P＞0.05),而病理亚型之间差异有统计学意义(P＜0.05),伴有B症状的HL患者多有EB病毒感染(P=0.02).EB病毒阳性HL患者中LMP2A蛋白阳性表达率为57.7％ (15/26),多在MC型及NS型中表达,而EB病毒阳性的淋巴结反应性增生组中未发现LMP2A蛋白表达.结论 维吾尔族HL患者EB病毒感染率较高,且与临床病理特征的关系存在自身特点,提示维吾尔族HL的发生与EB病毒相关.

abstractsObjective To investigate Epstein-Barr virus (EBV) infection in Hodgkin's lymphoma (HL) of Uygur patients and related clinicopathological characteristics.Methods EBV-encoded small RNA (EBER) was detected in 40 cases of HL and 20 cases of lymphoid reactive hyperplasia by in-situ hybridization.Expression of LMP2A in HL was investigated by immunohistochemistry.Results EBV was detected in 26/40(65.0％) of HL and 5/20 of lymphoid reactive hyperplasia (P ＜ 0.05).The expression level of EBER showed significant difference among various histological subtypes of HL (P ＜ 0.05) and between patients with and without B symptom (P =0.02).However,no difference was found in relation to gender,clinical stage and tumor burden.The expression of LMP2A in the mixed cellularity and nodular sclerosis classical HL associated with EBV infection was 57.7％ (15/26).Expression of LMP2A was not detected in lymphoid reactive hyperplasia cases.Conclusion Uyghur patients with Hodgkin's lymphoma have a high infection rate of EBV and distinct clinicopathologic characteristics.