摘要目的：研究两组同一分期预后显著不同的早期食管鳞状细胞癌（简称鳞癌）微小RNA （ miRNA）表达的差异，探索不同miRNA在早期食管鳞癌转移中的预后意义。方法采用TaqMan human microRNA 芯片技术分析预后显著不同的两组早期食管鳞癌381个miRNA表达情况。荧光定量PCR（ RT-PCR）方法验证差异miRNA表达结果。结果两组样本中共41个miRNA表达变化差异具有统计学意义（P＜0.05），数据经生物信息学分析、靶基因预测、筛选出靶基因显著性功能分析、信号通路分析，其中上调或下调7倍以上的miRNA为：miR-27a、miR-886-5p、miR-143。富集的靶基因为GRB2、SOS1、MAPK1、EGFR、CBL、SPRY2、RPS6KA5、IGF1R、NGFR、MAPK14、CREB1。这些基因与以下信号通路显著相关：Sprouty 调节的酪氨酸激酶信号通路、Erk1/Erk2丝裂原激活的蛋白激酶信号通路、转录因子CREB 和细胞外信号。结论 miR-27a、miR-886-5p、miR-143可能是早期食管鳞癌转移的潜在预后相关标志物，对早期食管鳞癌转移具有预测意义，这些标志物的检测对早期食管癌治疗方式选择具有指导意义。

abstractsObjective To study the difference of microRNA ( miRNA ) expression between two groups of early stage ( pT1N0 ) esophageal squamous cell carcinoma ( ESCC ) patients who had different outcome and the prognostic significance of different miRNA in metastatic of early ESCC , and to identify useful prognostic markers in the selection of appropriate treatment for early ESCC patients.Methods TaqMan human miRNA arrays and bioinformatics were used to detect and analyze the expression profiles of miRNAs in the two groups , and RT-PCR was used to verify the differences in miRNA expression.Results The miRNA arrays revealed a total of 41 markedly changed miRNAs in the survival group compared with the death group.Bioinformatics analysis , prediction and significant function analyses of targeted genes and pathway analysis identified that miR-27a, miR-143 and miR-886-5p levels were increased or decreased by seven-folds or more.The enriched target genes were GRB2, SOS1, MAPK1, EGFR, CBL, SPRY2, RPS6KA5, IGF1R, NGFR, MAPK14 and CREB1.These genes were significantly related to the following signaling pathways , i.e.Sprouty regulation of tyrosine kinase signals pathway , Erk1/Erk2 Mapk signaling pathway and transcription factor CREB and its extracellular signals.Conclusions miR-27a, miR-886-5p, and miR-143 may be potential prognostic markers of metastasis for early ESCC.The detection of these miRNAs plays a directive role for the treatment options of early ESCC.The regulation of targeted genes and mechanism remain to be further studied.