摘要目的 探讨醌氧化还原酶(NQO1)过表达在头颈部鳞状细胞癌(HNSCC)中的临床预后评估意义.方法 采用免疫组织化学EnVision法检测NQO1蛋白在162例HNSCC、45例癌旁非瘤上皮和26例正常头颈部上皮组织中的表达,并分析其过表达与HNSCC患者预后之间的关系.结果 HNSCC组织中NQO1蛋白阳性率和强阳性率分别高达84.0％(136/162)和69.8％ (113/162),均明显高于癌旁非瘤上皮和正常头颈部上皮组织(均P＜0.01);卡方检验结果显示,NQO1蛋白表达水平与HNSCC的临床分期、分化程度和pT分期均密切相关(均P＜0.01).Kaplan-Meier生存分析显示,NQO1蛋白高表达的HNSCC患者总生存期和无瘤生存期均明显低于NQO1蛋白低表达的患者(Log-rank分别为6.625和6.234,P分别为0.010和0.013).Cox风险回归模型分析结果显示,NQO1蛋白高表达是影响HNSCC患者预后的独立风险因素(Wald=6.626,P=0.008).结论 NQO1表达水平与HNSCC发生发展及预后密切相关.NQO1蛋白高表达可成为HNSCC患者不良预后的一个重要指标.

abstractsObjective To investigate the significance of NADPH quinine oxidoreductase 1 (NQO1) protein overexpression on prognostic evaluation of head and neck squamous cell carcinoma (HNSCC).Methods NQO1 protein was detected in 162 of HNSCC,45 cases of adjacent nontumor tissues and 26 samples of normal head and neck epithelia using EnVision immunohistochemical.Correlation between NQO1 overexpression and patients prognosis was also analyzed.Results The positive rate and strongly positive rate of NQO1 protein were 84.0％ (136/162) and 69.8％ (113/162) in HNSCC,respectively,and both of which were significantly higher than either those in adjacent nontumor tissues and normal head and neck epithelia (both P ＜ 0.01).NQO1 expression was significantly correlated with the clinical stage,pT and chemoradiotherapy of HNSCC (P ＜ 0.01).Kaplan-Meier survival analysis showed that overall survival and disease-free survival rates were significantly higher in HNSCC patients with high level NQO1 expression than that those with low level of NQO1 expression (Log-rank =6.625,P =0.010; Log-rank =6.234,P =0.013).Additional analysis by Cox proportional hazard regression model showed that high level of NQO1 expression was an independent hazard predictor for overall survival of patients with HNSCC (Wald =6.626,P =0.008).Conclusions NQO1 expression level is closely correlated with the progression and prognosis of patients with HNSCC.High level of NQO1 expression may be used as an important indicator for patients with poor prognostic HNSCC.