摘要目的 探讨Versican在胃癌组织中的表达及其与胃癌血管生成的关系.方法 用免疫组织化学EliVision方法检测80例患者胃癌组织和30例患者癌旁正常组织中Versican和血管内皮生长因子(VEGF)的表达,CD34标记胃癌的微血管密度(MVD).结果 (1)胃癌组织中的Versican、VEGF和MVD均显著高于正常胃黏膜(P＜0.05).(2)在胃癌中Versican主要表达于间质纤维母细胞的胞质,且其表达与胃癌的分化程度、临床分期及淋巴结转移相关(P＜0.05),VEGF主要表达于癌细胞的胞质,且与胃癌的分化程度、临床分期、Lauren分型及淋巴结转移显著相关(P＜0.05).(3)胃癌组织中的MVD与分化程度、临床分期、Lauren分型、浸润深度及淋巴结转移显著相关(P＜0.05).(4)在胃癌组织中Versican与VEGF的表达呈正相关(r=0.467,P＜0.01).结论 Versican和VEGF的表达与胃癌的血管生成关系密切,二者协同作用促进胃癌的血管生成.

abstractsObjective To investigate the expression of Versican in gastric carcinoma and its relationship with tumor angiogenesis.Methods Protein expression of Versican,vascular endothelial growth factor and CD34 was evaluated by immunohistochemistry (EliVision method) in 80 cases of gastric carcinoma and 30 samples of normal gastric tissue.Results There were statistically significant differences in the expression of Versican,vascular endothelial growth factor and CD34 between gastric carcinoma and normal gastric tissue (P ＜ 0.05).The expression of Versican was seen mainly in fibroblasts of the tumor and was correlated with tumor differentiation,clinical stage and lymph node metastasis (P ＜ 0.05),whereas vascular endothelial growth factor was primarily seen in the cytoplasm of the tumor cells and correlated with tumor differentiation,clinical stage,Lauren classification and lymph node metastasis (P ＜0.05).MVD was correlated with tumor differentiation,clinical stage,Lauren classification,depth of tumor invasion and lymph node metastasis(P ＜0.05).In addition,positive correlation of Versican and VEGF protein expression was found in tumor cells (r =0.467,P ＜ 0,01).Conclusion The expression of both Versican and vascular endothelial growth factor is closely associated with tumor angiogenesis in gastric carcinoma.