摘要目的 探讨Wnt通路中Sox17及β连环蛋白在少突胶质细胞起源肿瘤的表达及与临床病理特征的关系.方法 选择新疆医科大学第一附属医院2003至2013年间,经病理组织学诊断为不同级别的少突胶质细胞起源肿瘤合计100例,良性病变周围组织标本10例为对照、采用免疫组织化学EnVision法对10例对照和100例少突胶质细胞起源肿瘤Sox17及β连环素进行半定量分析,Kaplan-Meien和Cox回归分析Sox17、β连环蛋白与少突胶质细胞起源肿瘤临床病理特征及预后关系.结果 Sox17在正常脑组织中均有表达(10/10),少突胶质细胞瘤(WHOⅡ级)和间变性少突胶质细胞瘤(WHOⅢ级)中阳性率分别为82％ (41/50)、62％(31/50),三者间比较差异有统计学意义(P＜0.05);β连环蛋白阳性率分别为2/10、22％(11/50)、52％ (26/50),差异有统计学意义(P＜0.01);单因素分析Sox17蛋白(P=0.000)、β连环蛋白(P =0.033)、部位(P=0.001)、化疗(P=0.000)与少突胶质细胞瘤患者预后相关.结论 Sox17蛋白表达、β连环蛋白失表达者预后较好.联合检测少突胶质细胞起源肿瘤Sox17及β连环蛋白表达,有助于预后判断.

abstractsObjective To investigate the expression of Sox17 and β-catenin proteins in oligodendroglioma,and its clinical significance.Methods One hundred cases of oligodendroglioma of different grades and 10 cases of surrounding benign tissue from First Affiliated Hospital of Xinjiang Medical University from 2003 to 2013 were assessed by immunohistochemistry for Sox17 and β-catenin protein expression.The clinicopathologic characteristics and outcome of patients with oligodendroglioma were evaluated by Kaplan-Meien and Cox regression analyses.Results Sox17 was expressed in 10/10,82％ (41/50) and 62％ (31/50) of normal control,oligodendroglioma and anaplastic oligodendroglioma,respectively.β-catenin was expressed in 2/10,22％ (11/50),and 52％ (26/50) of normal control,oligodendroglioma and anaplastic oligodendroglioma,respectively.The differences of Sox17 and β-catenin expression between normal control and different types of oligodendroglioma were statistically significant.Univariate analysis showed that the expression of Sox17 protein (P =0.000),β-catenin protein (P =0.033),tumor position (P =0.001),radiotherapy (P =0.077),and chemotherapy (P =0.000) were significant prognostic factors.Conclusions Oligodendrogliomas with expression of Sox17 protein,but not β-catenin,have better prognosis.Evaluation of Sox17 and β-catenin protein expression is important for accurate pathological diagnosis,prognostication and guiding treatment.