摘要目的 探讨多基因组合荧光原位杂交(FISH)对皮肤恶性黑色素瘤的辅助诊断价值.方法 应用黑色素瘤多基因组合FISH检测试剂盒,对20例皮肤恶性黑色素瘤和20例良性皮肤黑色素细胞痣中3个特定基因位点(CCND1、RREB1、MYB)及第6号染色体着丝粒进行检测,并按试剂盒给出标准进行结果判读.结果 共有34例标本获得满意荧光杂交信号,可准确判读FISH结果.在恶性黑色素瘤病例组,16例可准确判读FISH结果,其中FISH阳性12例,FISH阴性4例.良性黑色素细胞痣组,18例可准确判读FISH结果,其中FISH阳性1例,阴性17例.FISH检测阳性率在恶性黑色素瘤病例组远大于良性黑色素细胞痣病例组(P＜0.01).结论 FISH检测对于皮肤恶性黑色素瘤的辅助诊断具有很好的特异性和较好的敏感性,有望成为皮肤黑色素瘤病理诊断和鉴别诊断的一种重要辅助方法.

abstractsObjective To explore the utility of fluorescence in situ hybridization as a diagnostic tool for cutaneous melanoma.Methods Twenty cutaneous melanomas and 20 cutaneous nevi from pathology files were selected and analyzed by Vysis melanoma FISH probe kit targeting 3 loci on chromosome 6 (MYB,CEP6 and RREB1) and 1 locus on 1 1q (CCND1) and data were interpreted based on the Abbott criteria provided by the kit.Results Informative FISH results were obtained in 16 melanomas and 18 nevi.Chromosomal aberrations were detected in 12 of the 16 melanomas and only 1 of 18 nevi.Conclusion FISH is a useful diagnostic tool and able to distinguish cutaneous nevus from melanoma with good sensitivity and specificity.