摘要目的：探讨类表皮生长因子域7（EGFL7）对内皮细胞迁移和血管生成的影响。方法以人微血管内皮细胞为研究对象，构建EGFL7过表达载体，转染入细胞。应用即时定量PCR法检测EGFL7 mRNA表达水平及Western blot检测EGFL7蛋白表达水平。分别采用划痕实验法、Matrigel法观察EGFL7对内皮细胞迁移及血管生成的影响。 Western blot检测内皮细胞转染EGFL7过表达载体和空载体0、10、30和60 min后，p－AKT、AKT、p－ERK和ERK蛋白表达情况。结果 EGFL7过表达组可以显著促进内皮细胞的迁移、血管生成；EGFL7可以激活ERK信号通路，但对AKT信号通路几乎无影响；抑制 ERK 信号通路后， EGFL7对内皮细胞迁移和血管生成的影响均显著下降。结论EGFL7通过ERK信号通路影响内皮细胞迁移和血管生成。

abstractsObjective To explore the effect of epidermal growth factor-like domain 7 ( EGFL7 ) on the migration and angiogenesis of endothelial cells.Methods EGFL7 overexpression vectors were constructed and transfected into human microvascular endothelial cells.The expression levels of EGFL7-mRNA and EGFL7 protein were examined by real-time RT-PCR and Western blot.Cell migration was analyzed by the wound healing.The capability of cell to form capillary-like tubes in vitro was evaluated on matrigel assay.Protein expression of p-AKT, AKT, p-ERK and ERK in endothelial cells was detected by Western blot upon transfection with EGFL7 overexpression vectors and vehicle control for 0, 10, 30 and 60 min. Results Migration and angiogenesis of endothelial cells were notably enhanced by EGFL7 overexpression.ERK pathway was strongly activated by EGFL7, whereas AKT remained constant in endothelial cells.Inhibition of ERK impaired EGFL7 induced ERK activation and endothelial cell migration and angiogenesis. Conclusion EGFL7 effectively promotes migration and angiogenesis through ERK signaling pathway in endothelial cells.