摘要目的 探讨胰腺神经内分泌肿瘤中ARID 1A编码的BAF250蛋白质的表达情况,及ARID1A在胰腺神经内分泌肿瘤中的作用.方法 收集2003年7月至2012年5月北京协和医院所收治的胰腺神经内分泌肿瘤51例和含有正常胰岛的胰腺组织18名,采用免疫组织化学EnVision法染色检测神经内分泌肿瘤中ARID1A的表达情况,分析ARID 1A表达与肿瘤分级、分期的关系.结果 ARID1A表达水平在不同性别、年龄、TNM分期、肿瘤大小分组中差异均无统计学意义(P＞0.05);ARID1A染色评分与肿瘤分级呈负相关(P＜0.05),肿瘤级别越高,ARIDIA染色越浅,蛋白表达越少,部分区域呈阴性表达.正常胰腺组织中表达高于肿瘤组织.结论 ARID1A蛋白表达与胰腺神经内分泌肿瘤分级呈负相关,ARID1A可能发挥着抑癌基因的作用.

abstractsObjective To study ARID1A expression and its role in pancreatic neuroendocrine tumors.Methods 51 cases of pancreatic neuroendocrine tumors were collected from July 2003 to May 2012 from Peking Union Medical College Hospital.Eighteen normal pancreatic tissues were used as positive control.HE staining method was used to observe general clinicopathological features.ARID1A expression was detected immunohistochemically by EnVision method.The relationship between ARID1A expression and tumor grade and stage was analyzed.Results The level of ARID1A expression was not associated with patients' gender,age,TNM stage,and tumor size (P ＞ 0.05);but showed significantly negative correlation with tumor grade (P ＜ 0.05).Conclusions Low ARID1A expression was significantly related to the high grade level of pancreatic neuroendocrine tumors.It has a certain effect on the clinical classification of pancreatic neuroendocring tumors.ARID1A may play a role as a tumor suppressor gene.