摘要目的：探讨miR－93对骨肉瘤细胞增殖和凋亡的影响及其可能的机制。方法采用即时定量PCR法检测骨肉瘤细胞株143B、HuO9、Saos2、MG63、U2OS和G292和人成骨细胞系hFOB1．19中miR－93的表达水平，免疫印迹法检测裸露角质蛋白2（ NKD2）的表达水平。脂质体转染构建miR－93低表达的143B和HuO9细胞，四甲基偶氮唑盐比色法检测转染后细胞的增殖情况，流式细胞术检测其凋亡情况。利用荧光素酶报告基因实验检测miR－93对NKD2的靶向作用，过表达NKD2，分析NKD2对骨肉瘤细胞增殖和凋亡的影响。结果 miR－93在骨肉瘤细胞系中明显高表达，NKD2显著低表达。转染miR－93抑制剂后明显抑制了143B和HuO9细胞的增殖，促进其凋亡。荧光素酶实验证实NKD2是 miR－93的直接靶基因， NKD2过表达抑制骨肉瘤细胞增殖，促进其凋亡。结论miR－93通过靶向抑制NKD2的表达促进骨肉瘤细胞增殖，抑制其凋亡，这对更深入探索骨肉瘤的诊断和治疗有着重要意义。

abstractsObjective To investigate the effects of miR-93 on proliferation and apoptosis of osteosarcoma cells and the possible mechanism .Methods The expression levels of miR-93 and the naked cuticle homolog 2 (NKD2) in 6 osteosarcoma cell lines (143B, HuO9, Saos2, MG63, U2OS and G292) and one osteoblast cell line hFOB 1.19 were determined by quantitative real-time PCR and Western blot assays, respectively. MiR-93 down-regulated 143B and HuO9 cells were constructed by lipofection transfection, and their proliferation and apoptosis were detected by MTT and flow cytometry assays , respectively.Luciferase reporter assay was used to determine whether the 3′UTR of NKD2 mRNA was a binding target of miR-93.In addition, 143B cells were transfected with NKD2 cDNA, and the effects of NKD2 on proliferation and apoptosis of osteosarcoma cells were investigated .Results Up-regulation of miR-93 and down-regulation of NKD2 were detected in osteosarcoma cell lines .MTT and flow cytometry assays showed that miR-93 promoted proliferation and inhibited apoptosis in osteosarcoma cells .Luciferase assay confirmed that miR-93 targeted NKD2 directly. In addition, overexpression of NKD2 inhibited proliferation and promoted apoptosis of osteosarcoma cells were found .Conclusions MiR-93 targets NKD2 to promote proliferation and inhibit apoptosis of osteosarcoma cells . The findings may have significant implications in the diagnosis and treatment of osteosarcoma .