摘要目的探讨缺氧诱导因子1α（ HIF-1α）、葡萄糖转运蛋白1（ GLUT-1）及乳酸脱氢酶5（LDH-5）在结直肠癌中的表达及其临床病理意义。方法采用免疫组织化学SP法检测142例结直肠癌组织及其癌旁组织中HIF-1α、GLUT-1及LDH-5蛋白的表达。结果 HIF-1α、GLUT-1及LDH-5在结直肠癌组织的表达率分别为78.2％（111／142）、75.4％（107／142）及68.3％（97／142），与癌旁组织的表达率（14.8％，21／142；11.3％，16／142；7.0％，10／142）比较，其差异均具有统计学意义（ P＜0.01）。结直肠癌组织中HIF-1α、GLUT-1及LDH-5的阳性表达率与淋巴结转移、肿瘤分化程度和病理分期有相关性（均P＜0.05）；结直肠癌中HIF-1α、GLUT-1和LDH-5的表达两两之间具有关联性，并且表达呈正相关（ P＜0.05）。结论联合检测HIF-1α、GLUT-1及LDH-5在结直肠癌的表达，可作为评估结直肠癌的发展、转移及预后重要指标。

abstractsObjective To investigate the expression and clinicopathological significance of hypoxia -inducible factor 1 alpha (HIF-1α), glucose transporter 1(GLUT-1) and lactate dehydrogenase(LDH)-5 in colorectal cancer .Methods The expression levels of HIF-1α, GLUT-1 and LDH-5 were detected by immunohistochemical staining in 142 specimens of human carcinoma in comparison with adjacent normal tissues.Results The expression levels of HIF-1α( 78.2%,111/142 ) , GLUT-1 ( 75.4%,107/142 ) and LDH-5(68.3%,97/142) were higher in tumor tissues than in adjacent normal tissues (14.8%,21/142;11.3%,16/142;7.0%,10/142;P<0.01 for all three proteins ) , and such over-expression was significantly associated with lymphovascular invasion , tumor grade and pathological stages ( all P<0.01 ).Additional studies showed that HIF-1α, GLUT-1 and LDH-5 were positively associated with each other ( r<0.3, P<0.05 for all three proteins ) .Conclusion The data suggest that HIF-1α, GLUT-1 and LDH-5 expression may serve as prognostic indicators for colorectal cancer patients.