摘要目的 探讨ERG、Fli-1、CD34、CD31、第八因子相关抗原(FⅧRAg)在肝脏血管源性恶性肿瘤中的表达特征及意义.方法 对解放军总医院病理科1986年1月至2014年1月检出的63例肝脏血管源性恶性肿瘤及31例肝脏其他恶性肿瘤,采用免疫组织化学EnVision法检测ERG、Fli-1、CD34、CD31、FⅧRAg的表达情况.结果 63例肝脏血管源性恶性肿瘤包括24例血管肉瘤、38例上皮样血管内皮瘤和1例Kaposi肉瘤.其中,ERG表达率为100.0%(63/63),Fli-1表达率为96.8%(61/63),CD34表达率为87.3%(55/63),CD31表达率为81.0%(51/63),FⅧRAg表达率为41.3%(26/63).在31例肝脏其他恶性肿瘤中,1例表达ERG(3.2%),6例表达Fli-1(19.4%),6例表达CD34(19.4%),3例表达CD31(9.7%),1例表达FⅧRAg(3.2%).肝脏血管源性恶性肿瘤中,ERG、Fli-1、CD34、CD31、FⅧRAg的灵敏度分别为100.0%、96.8%、87.3%、81.0%和41.3%,特异度分别为96.8%、80.6%、80.6%、90.3%和96.8%.结论 在诊断肝内血管来源恶性肿瘤的各种标志物中, ERG的灵敏度和特异度优于Fli-1、CD34、CD31和FⅧRAg,有一定的诊断价值.

abstractsObjective To investigate the expression of ERG, Fli-1,CD34,CD31 and factorⅧ-related antigen(FⅧRAg)in hepatic malignant vascular tumors. Methods A retrospective analysis was conducted on 63 cases of primary hepatic malignant vascular tumors and 31 cases of hepatic other malignant spindle cell tumors collected during January 1986 to January 2014. EnVision method was used to detect the expression of ERG,Fli-1,CD34,CD31,FⅧRAg. Results Sixty-three cases of malignant vascular tumors, including 24 cases of angiosarcoma, 38 cases of epithelioid hemangioendothelioma and 1 case of hepatic Kaposi′s sarcoma. All of the cases were positive for ERG(100.0%,63/63). Positive rate of Fli-1,CD34, CD31,FⅧRAg was 96.8%(61/63),87.3%(55/63),81.0%(51/63)and 41.3%(26/63), respectively. In other hepatic malignant spindle cell tumors,the positive rate of ERG,Fli-1, CD34, CD31 and FⅧRAg was 3.2%(1/31), 19.4%(6/31),19.4%(6/31), 9.7%(3/31)and 3.2%(1/31), respectively.The sensitivity of ERG,Fli-1,CD34,CD31,FⅧRAg was 100.0%,96.8%,87.3%,81.0% and 41.3%, respectively.The specificity was 96.8%,80.6%,80.6%,90.3% and 96.8%,respectively. Conclusion ERG is a more sensitive and specific diagnostic marker for hepatic malignant vascular tumors in comparison to Fli-1,CD34,CD31 and FⅧRAg.