摘要目的 探讨弥漫性大B细胞淋巴瘤(DLBCL)中癌基因C-MYC蛋白表达及与基因异常的相关性.方法 回顾性收集山东省潍坊市人民医院病理科2015年1月至2016年10月首次诊断为DLBCL的石蜡包埋组织标本42例,采用免疫组织化学和荧光原位杂交(FISH)技术检测全部研究对象组织标本中C-MYC蛋白表达与基因异常的情况,观察两者之间的相关性.结果 C-MYC蛋白表达阳性率为47.6%(20/42);C-MYC 基因异常检出率为26.2%(11/42),其中基因易位检出率23.8%(10/42);基因扩增2.4%(1/42);C-MYC蛋白表达阳性与 C-MYC 基因易位有相关性(χ2=11.813;P=0.001);C-MYC 基因易位主要见于生发中心 B 细胞型(χ2=4.029;P=0.045).结论 C-MYC蛋白表达阳性(≥40%)与C-MYC基因易位有相关性.在DLBCL中,C-MYC蛋白高表达提示可能存在C-MYC基因易位.

abstractsObjective To study the correlation between expression of oncogene C-MYC protein and gene abnormality in diffuse large B-cell lymphoma(DLBCL). Methods The expression of C-MYC protein and gene abnormality were detected by immunohistochemistry and fluorescence in situ hybridization(FISH), respectively,in 42 cases of paraffin-embedded DLBCL. All cases were collected at Department of Pathology, Weifang People′s Hospital during January 2015 to October 2016. Results The positive rate of C-MYC protein expression was 47.6%(20/42)and the rate of abnormal C-MYC gene by FISH was 26.2%(11/42),including translocation(23.8%, 10/42)and gene amplification(2.4%, 1/42).There was a close relationship between the protein expression and gene translocation(χ2=11.813;P=0.001)and gene translocation occurred primarily in GCB(χ2 =4.029; P=0.045). Conclusion The high expression (≥40%)of C-MYC protein is associated with its gene translocation, suggesting that C-MYC protein detection can be used as a surrogate marker for C-MYC gene translocation in DLBCL.