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Unilateral amyloid-β-25-35 injection into the rat amygdala increases the expressions of aberrant tau phosphorylation kinases

摘要:

Background Neuropathologically, Alzheimer disease (AD) is characterized by the presence of extracellular plaques enriched in β-amyloid peptides; however, the mechanism by which it results in the neurotoxicity is uncertain.The purpose of this study was to investigate whether it would prompt the progress of Alzheimer disease via enhancement of aberrant phosphorylated tau that results from its increased kinase gene expression.Methods Twenty-four male rats were divided into three groups, and each group had 8 rats:control, sham-operated,and Aβ25-35 injected AD model groups.AD rat models were created by unilateral injections of Aβ25-35 into the amygdala.The hyperphosphorylated tau protein was estimated by immunohistochemistry with paired helical filament-1 (PHF-1)antibody and paired helical filament-tau (AT8) antibody.The expressions of glycogen synthase kinase-3β (GSK-3β) and p38 mitogen-activated protein kinase (P38MAPK) mRNA were observed by in situ hybridization.Results Compared with the control and sham-operated groups, the evaluation of paired AT8 and paired helical filament-1 (PHF-1) in the cortexes and hippocampus of the AD model group showed the numbers of AT8 and PHF-1 positive cells, as well as the optical density (OD) values of the proteins were significantly higher (AT8:in CA2:0.318±0.037 vs.0.135±0.028, 0.136±0.031; in frontal cortex:0.278±0.040 vs.0.130±0.028, 0.190±0.037.PH F-1:in CA2:0.386±0.034 vs.0.139±0.010, 0.193±0.041; in frontal cortex:0.395±0.050 vs.0.159±0.030, 0.190±0.044, respectively, P <0.01); the number of GSK-3β mRNA and P38MAPK mRNA positive cells of the AD model group, as well as the OD values, also increased significantly in the cortexes, hippocampus (GSK-3β-mRNA:in CA2:0.384±0.012 vs.0.190±0.015,0.258±0.064; in frontal cortex:0.398±0.018 vs.0.184±0.031, 0.218±0.049.P38MAPK mRNA:in CA2:0.409±0.038 vs.0.161±0.041,0.189±0.035; in frontal cortex:0.423±0.070 vs.0.160±0.032, 0.203±0.053, respectively, P <0.01).Conclusion Unilateral injection of Aβ25-35 into the rat amygdala increases the generation of aberrant phosphorylated tau by increasing GSK-3β and P38MAPK gene expression, that accelerates the process of Alzhemer's disease.

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期刊: 《中华医学杂志(英文版)》2010年123卷10期 1311-1314页 SCIMEDLINEISTICCSCDBP
分类号: R5
栏目名称: ORIGINAL ARTICLES
DOI: 10.3760/cma.j.issn.0366-6999.2010.10.016
发布时间: 2010-06-29
基金项目:
the grants from the National Natural Science Foundation of China Fund of Shanghai Science and Technology Committee Young Excellent Talents Award of Tongji University
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